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Product Info Summary
| SKU: | A03641 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Chimpanzee |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-GGA3 Antibody
SKU/Catalog Number
A03641
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-GGA3 Antibody (Catalog # A03641). Tested in ELISA, WB applications. This antibody reacts with Human, Chimpanzee.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-GGA3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03641)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 400-425 of Human GGA3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03641 is reactive to GGA3 in Human, Chimpanzee
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
42 kDa
Calculated molecular weight
93886 MW
Background of GGA3
GGA3 (also known as ADP-ribosylation factor binding protein GGA3, Golgi-localized, gamma ear-containing, ARF-binding protein 3, Gamma-adaptin related protein 3) plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. This protein mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. GGA1 is a monomeric protein that interacts with SORT1, SORL1, LRP3, GGA binding partner (GGABP) and P200, GGA1 and GGA2 as well as RABEP1, RABGEF1 and type-I membrane proteins M6PR/CD-MPR, IGF2R/CI-MPR and BACE1. GGA3 is a ubiquitously expressed membrane-associated protein that is localized to TGN and endosomes. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03641 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:50,000
IP: 1:100
WB: 1:500 - 1:3,000
Validation Images & Assay Conditions
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ELISA results of purified Rabbit anti-GGA3 Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1
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Figure 1. Western blot analysis of GGA3 using anti-GGA3 antibody (A03641).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GGA3 antigen affinity purified polyclonal antibody (Catalog # A03641) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for GGA3.
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Figure 3. Western blot analysis of GGA3 using anti-GGA3 antibody (A03641).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GGA3 antigen affinity purified polyclonal antibody (Catalog # A03641) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for GGA3.
Protein Target Info & Infographic
Gene/Protein Information For GGA3 (Source: Uniprot.org, NCBI)
Gene Name
GGA3
Full Name
ADP-ribosylation factor-binding protein GGA3
Weight
93886 MW
Superfamily
GGA protein family
Alternative Names
GGA3, GGA-3, GGA 3, ADP-ribosylation factor-binding protein GGA3, Golgi-localized gamma ear-containing ARF-binding protein 3 GGA3 golgi associated, gamma adaptin ear containing, ARF binding protein 3 ADP-ribosylation factor-binding protein GGA3|Golgi-localized, gamma ear-containing, ARF-binding protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GGA3, check out the GGA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GGA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GGA3 Antibody (A03641)
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4 Customer Q&As for Anti-GGA3 Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow using anti-GGA3 antibody A03641. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-14
Question
We are currently using anti-GGA3 antibody A03641 for human tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
The anti-GGA3 antibody (A03641) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-08
Question
I was wanting to use to test anti-GGA3 antibody A03641 on human bone marrow for research purposes, then I may be interested in using anti-GGA3 antibody A03641 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
The products we sell, including anti-GGA3 antibody A03641, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-17
Question
Do you have a BSA free version of anti-GGA3 antibody A03641 available?
O. Krishna
Verified customer
Asked: 2014-02-03
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GGA3 antibody A03641 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-02-03
