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Product Info Summary
| SKU: | PB9202 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-GDNF Receptor alpha 1/GFRA1 Antibody Picoband®
View all GFR alpha-1/GDNF R alpha-1 Antibodies
SKU/Catalog Number
PB9202
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GDNF Receptor alpha 1/GFRA1 Antibody Picoband® catalog # PB9202. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GDNF Receptor alpha 1/GFRA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9202)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GFRA1 recombinant protein (Position: D25-Q227). Human GFRA1 shares 97% amino acid (aa) sequence identity with both mouse and rat GFRA1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9202 is reactive to GFRA1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
51 kDa
Calculated molecular weight
51456 MW
Background of GFR alpha-1/GDNF R alpha-1
GDNF family receptor alpha-1 (GFRα1), also known as the GDNF receptor or GFRA1, is a protein that in humans is encoded by the GFRA1 gene. It is mapped to chromosome 10q25.3. The protein encoded by this gene is a member of the GDNF receptor family. GFRA1 is released by neuronal cells, Schwann cells, and injured sciatic nerve. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is also a candidate gene for Hirschsprung disease. Soluble GFRA1 mediates robust recruitment of RET to lipid rafts via a mechanism requiring the RET tyrosine kinase.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9202 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human placenta tissue,
IHC: human lung cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GFRA1 using anti-GFRA1 antibody (PB9202).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human GFRA1 protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GFRA1 antigen affinity purified polyclonal antibody (Catalog # PB9202) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GFRA1 at approximately 39 kDa. The expected band size for GFRA1 is at 39 kDa.
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Figure 2. Western blot analysis of GFRA1 using anti-GFRA1 antibody (PB9202).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GFRA1 antigen affinity purified polyclonal antibody (Catalog # PB9202) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GFRA1 at approximately 51 kDa. The expected band size for GFRA1 is at 51 kDa.
Click image to see more details
Figure 3. IHC analysis of GFRA1 using anti-GFRA1 antibody (PB9202).
GFRA1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GFRA1 Antibody (PB9202) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For GFRA1 (Source: Uniprot.org, NCBI)
Gene Name
GFRA1
Full Name
GDNF family receptor alpha-1
Weight
51456 MW
Superfamily
GDNFR family
Alternative Names
GDNF family receptor alpha-1;GDNF receptor alpha-1;GDNFR-alpha-1;GFR-alpha-1;RET ligand 1;TGF-beta-related neurotrophic factor receptor 1;GFRA1;GDNFRA, RETL1, TRNR1; GFRA1 GDNFR, GDNFRA, GFR-ALPHA-1, GFRalpha-1, RET1L, RETL1, TRNR1 GDNF family receptor alpha 1 GDNF family receptor alpha-1|GDNF receptor alpha 1d|GDNF receptor alpha 1e|GDNFR-alpha-1|GPI-linked anchor protein|Glial cell line-derived neurotrophic factor receptor alpha|PI-linked cell-surface accessory protein|RET ligand 1|TGF-beta-related neurotrophic factor receptor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GFRA1, check out the GFRA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GFRA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GDNF Receptor alpha 1/GFRA1 Antibody Picoband® (PB9202)
Hello CJ!
PB9202 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhu WQ,Cai NN,Jiang Y,Yang R,Shi JZ,Zhu CL,Zhang BY,Tang B,Zhang XM.Survivable potential of germ cells after trehalose cryopreservation of bovine testicular tissues.Cryobiology.2021 May 11:S0011-2240(21)00081-X.doi:10.1016/j.cryobiol.2021.05.001.Epub ahead of print.PMID:33989617.
Species: Bull
PB9202 usage in article: APP:IF, SAMPLE:TESTICULAR TISSUE, DILUTION:1:400
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Customer Q&As
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4 Customer Q&As for Anti-GDNF Receptor alpha 1/GFRA1 Antibody Picoband®
Question
We are currently using anti-GDNF Receptor alpha 1/GFRA1 antibody PB9202 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
The anti-GDNF Receptor alpha 1/GFRA1 antibody (PB9202) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-17
Question
Will PB9202 anti-GDNF Receptor alpha 1/GFRA1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
It shows on the product datasheet, PB9202 anti-GDNF Receptor alpha 1/GFRA1 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-21
Question
Do you have a BSA free version of anti-GDNF Receptor alpha 1/GFRA1 antibody PB9202 available?
D. Krishna
Verified customer
Asked: 2019-04-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GDNF Receptor alpha 1/GFRA1 antibody PB9202 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-04-26
Question
Would anti-GDNF Receptor alpha 1/GFRA1 antibody PB9202 work on primate IHC with substantia nigra?
Verified Customer
Verified customer
Asked: 2017-12-25
Answer
Our lab technicians have not validated anti-GDNF Receptor alpha 1/GFRA1 antibody PB9202 on primate. You can run a BLAST between primate and the immunogen sequence of anti-GDNF Receptor alpha 1/GFRA1 antibody PB9202 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate substantia nigra in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-25
