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Product Info Summary
| SKU: | A03180 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-GFI1B Antibody (C-term)
SKU/Catalog Number
A03180
Size
80 µl
Description
Boster Bio Anti-GFI1B Antibody (C-term) (Catalog # A03180). Tested in WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-GFI1B Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03180)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This GFI1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-330 amino acids from the C-terminal region of human GFI1B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03180 is reactive to GFI1B in Human, Mouse
Reconstitution
Calculated molecular weight
37492 Da
Background of GFI1B
Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Component of a RCOR-GFI-KDM1A-HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03180 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
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GFI1B Antibody (C-term) western blot analysis in HL-60,K562,U266
cell line and mouse spleen lysates (35ug/lane). This demonstrates the GFI1B antibody detected the GFI1B protein (arrow).
Protein Target Info & Infographic
Gene/Protein Information For GFI1B (Source: Uniprot.org, NCBI)
Gene Name
GFI1B
Full Name
Zinc finger protein Gfi-1b
Weight
37492 Da
Alternative Names
Zinc finger protein Gfi-1b, Growth factor independent protein 1B, Potential regulator of CDKN1A translocated in CML, GFI1B GFI1B BDPLT17, ZNF163B growth factor independent 1B transcriptional repressor zinc finger protein Gfi-1b|growth factor independent 1B (potential regulator of CDKN1A, translocated in CML)|growth factor independent 1B transcription repressor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GFI1B, check out the GFI1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GFI1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GFI1B Antibody (C-term) (A03180)
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