Product Info Summary
SKU: | M02948 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-GCLM Monoclonal Antibody
SKU/Catalog Number
M02948
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GCLM Monoclonal Antibody catalog # M02948. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GCLM Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02948)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEOH-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GCLM Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02948 is reactive to GCLM in Human, Mouse, Rat
Applications
M02948 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
59 kDa
Calculated molecular weight
56560 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:100
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of GCLM expression in K562 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For GCLM (Source: Uniprot.org, NCBI)
Gene Name
GCLM
Full Name
Glutamate--cysteine ligase regulatory subunit
Weight
56560 MW
Superfamily
aldo/keto reductase family
Alternative Names
Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; GCS light chain; GLCLRglutamate--cysteine ligase regulatory subunit; glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory(30.8kD); Glutamate--cysteine ligase modifier subunit; glutamate-cysteine ligase regulatory protein; glutamate-cysteine ligase, modifier subunit; GSC light chain GCLM GLCLR glutamate-cysteine ligase modifier subunit glutamate--cysteine ligase regulatory subunit|GCS light chain|GSC light chain|gamma-ECS regulatory subunit|gamma-glutamylcysteine synthetase regulatory subunit|glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory (30.8kD)|glutamate-cysteine ligase modifier subunit delta2 alternative splicing|glutamate-cysteine ligase regulatory protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GCLM, check out the GCLM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GCLM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GCLM Monoclonal Antibody (M02948)
Hello CJ!
No publications found for M02948
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-GCLM Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-GCLM Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-GCLM Monoclonal Antibody
Question
We are currently using anti-GCLM Monoclonal antibody M02948 for human tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
The anti-GCLM Monoclonal antibody (M02948) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-31
Question
See below the WB image, lot number and protocol we used for nasal cavity epithelium using anti-GCLM Monoclonal antibody M02948. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-26
Question
I have a question about product M02948, anti-GCLM Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
H. Wu
Verified customer
Asked: 2017-06-22
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02948 anti-GCLM Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-22