Anti-Gbp4 Antibody Picoband™

Product Name

Anti-Gbp4 Antibody Picoband™

View all Gbp4 Antibodies

SKU/Catalog Number

A11011-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Gbp4 Antibody Picoband™ catalog # A02860-4. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Gbp4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11011-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Gbp4 recombinant protein (Position: E9-G277).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A11011-3 is reactive to Gbp4 in Mouse

Applications

A11011-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

73 kDa

Calculated molecular weight

Background of Gbp4

Guanylate binding protein 4 is a protein in humans that is encoded by the GBP4 gene. Guanylate-binding protein 4, Binds GTP, GDP and GMP. Hydrolyzes GTP very efficiently; GDP rather than GMP is the major reaction product. Plays a role in erythroid differentiation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
Direct ELISA, 0.1-0.5 μg/ml, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Gbp4 using anti-Gbp4 antibody (A11011-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gbp4 antigen affinity purified polyclonal antibody (Catalog # A11011-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gbp4 at approximately 73 kDa. The expected band size for Gbp4 is at 73 kDa.

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Figure 2. Flow Cytometry analysis of ANA-1 cells using anti-Gbp4 antibody (A11011-3).
Overlay histogram showing ANA-1 cells stained with A11011-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Gbp4 Antibody (A11011-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For Gbp4 (Source: Uniprot.org, NCBI)

Alternative Names

GBP-4; GTP-binding protein 4; Guanine nucleotide-binding protein 4; guanylate binding protein 4; guanylate-binding protein 4; Mpa2

For more info on Gbp4, check out the Gbp4 Infographic

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In this infographic, you will see the following information for Gbp4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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