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Product Info Summary
| SKU: | M04694 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IF, IHC, WB |
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Product info
Product Name
Anti-GBE1 Mouse Monoclonal Antibody [Clone ID: OTI1D11]
SKU/Catalog Number
M04694
Size
100 µl
Description
Boster Bio GBE1 mouse monoclonal antibody, clone OTI1D11 (formerly 1D11). Catalog# M04694. Tested in FC, IF, IHC, IP, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-GBE1 Mouse Monoclonal Antibody [Clone ID: OTI1D11] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04694)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1D11
Isotype
IgG1
Immunogen
Full length human recombinant protein of human GBE1 (NP_000149) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04694 is reactive to GBE1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
80.474kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04694 is guaranteed for Flow Cytometry, IP, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1.
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Figure from citation: Western Blot of GBE protein level by using anti-GBE antibody in mouse muscle extracts obtained from Gbe1-/-. Gbe1+/-and Gbe1+/+ embryos.
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-GBE1 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-GBE1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either GBE1 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GBE1 antibody (M04694)
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Flow cytometric Analysis of Jurkat cells
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Anti-GBE1 mouse monoclonal antibody (M04694) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GBE1.
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Immunofluorescent staining of HepG2 cells using anti-GBE1 mouse monoclonal antibody (M04694).
Protein Target Info & Infographic
Gene/Protein Information For GBE1 (Source: Uniprot.org, NCBI)
Gene Name
GBE1
Full Name
1,4-alpha-glucan-branching enzyme
Weight
80.474kDa
Superfamily
glycosyl hydrolase 13 family
Alternative Names
1,4-alpha-glucan-branching enzyme; amylo-(1,4 to 1,6) transglucosidase; amylo-(1,4 to 1,6) transglycosylase; Brancher enzyme; EC 2.4.1.18; GBE; glucan (1,4-alpha), branching enzyme 1; glycogen branching enzyme; Glycogen-branching enzyme GBE1 APBD, GBE, GSD4 1,4-alpha-glucan branching enzyme 1 1,4-alpha-glucan-branching enzyme|amylo-(1,4 to 1,6) transglucosidase|amylo-(1,4 to 1,6) transglycosylase|brancher enzyme|glucan (1,4-alpha-), branching enzyme 1|glycogen branching enzyme
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GBE1, check out the GBE1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GBE1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GBE1 Mouse Monoclonal Antibody [Clone ID: OTI1D11] (M04694)
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