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Product Info Summary
| SKU: | A08549-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-GBA3 Antibody Picoband®
View all Cytosolic beta-Glucosidase/GBA3 Antibodies
SKU/Catalog Number
A08549-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-GBA3 Antibody Picoband® catalog # A08549-2. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-GBA3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08549-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human GBA3 recombinant protein (Position: Q17-L469).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08549-2 is reactive to GBA3 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
53.696kDa
Background of Cytosolic beta-Glucosidase/GBA3
Cytosolic beta-glucosidase, also known as cytosolic beta-glucosidase-like protein 1, is a beta-glucosidase (EC 3.2.1.21) enzyme that in humans is encoded by the GBA3 gene. The protein encoded by this gene is a cytosolic enzyme that can hydrolyze several types of glycosides. The enzyme has its highest activity at neutral pH and is predominantly expressed in human liver, kidney, intestine, and spleen. This gene is a polymorphic pseudogene, with the most common allele being the functional allele that encodes the full-length protein. Some individuals contain a single nucleotide polymorphism that results in a premature stop codon in the coding region, and therefore this allele is pseudogenic due to the failure to produce a functional full-length protein. Alternative splicing of this gene results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08549-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Mouse, Rat
ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: rat liver tissue, rat kidney tissue, mouse liver tissue, mouse kidney tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of GBA3 using anti-GBA3 antibody (A08549-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: mouse liver tissue lysates,
Lane 4: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GBA3 antigen affinity purified polyclonal antibody (Catalog # A08549-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GBA3 at approximately 50 kDa. The expected band size for GBA3 is at 54 kDa.
Protein Target Info & Infographic
Gene/Protein Information For GBA3 (Source: Uniprot.org, NCBI)
Gene Name
GBA3
Full Name
Cytosolic beta-glucosidase
Weight
53.696kDa
Superfamily
glycosyl hydrolase 1 family
Alternative Names
CBG; CBGL1; CBGL1GLUC; Cytosolic betaGlucosidase; Cytosolic beta-Glucosidase; Cytosolic beta-glucosidase-like protein 1; EC 3.2.1.21; GBA3; GLUC; glucosidase, beta, acid 3 (cytosolic); klotho-related protein; KLrP; MGC104276; MGC126878 GBA3 CBG, CBGL1, GLUC, KLRP glucosylceramidase beta 3 (gene/pseudogene) cytosolic beta-glucosidase|cytosolic GCase|cytosolic beta-glucosidase-like protein 1|cytosolic glycosylceramidase|glucosidase beta acid 3|glucosidase, beta, acid 3 (cytosolic)|klotho-related protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GBA3, check out the GBA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GBA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GBA3 Antibody Picoband® (A08549-2)
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