Anti-GBA2 Antibody Picoband®

Product Name

Anti-GBA2 Antibody Picoband®

View all GBA2 Antibodies

SKU/Catalog Number

A08047-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GBA2 Antibody Picoband® catalog # A08047-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-GBA2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08047-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human GBA2 recombinant protein (Position: E17-Q869). Human GBA2 shares 88.5% and 90.2% amino acid (aa) sequence identity with mouse and rat GBA2, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08047-1 is reactive to GBA2 in Human, Mouse, Rat

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

105 kDa

Calculated molecular weight

104.649kDa

Background of GBA2

GBA2 is the gene that encodes the enzyme non-lysosomal glucosylceramidase in humans. This gene encodes a microsomal beta-glucosidase that catalyzes the hydrolysis of bile acid 3-O-glucosides as endogenous compounds. Studies to determine subcellular localization of this protein in the liver indicated that the enzyme was mainly enriched in the microsomal fraction where it appeared to be confined to the endoplasmic reticulum. This putative transmembrane protein is thought to play a role in carbohydrate transport and metabolism.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08047-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, Human

Positive Control

WB: human HepG2 whole cell, human SH-SY5Y whole cell, human PC-3 whole cell, rat brain tissue, mouse brain tissue
FCM: A549 cell

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of GBA2 using anti-GBA2 antibody (A08047-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GBA2 antigen affinity purified polyclonal antibody (Catalog # A08047-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GBA2 at approximately 105 kDa. The expected band size for GBA2 is at 105 kDa.

Click image to see more details

Figure 2. Flow Cytometry analysis of A549 cells using anti-GBA2 antibody (A08047-1).
Overlay histogram showing A549 cells stained with A08047-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GBA2 Antibody (A08047-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For GBA2 (Source: Uniprot.org, NCBI)

Alternative Names

AD035; Beta-glucocerebrosidase 2; Beta-glucosidase 2; bile acid beta-glucosidase; DKFZp762K054; glucosidase, beta (bile acid) 2; Glucosylceramidase 2; KIAA1605EC 3.2.1.45; MGC16895; NLGase; non-lysosomal glucosylceramidase GBA2 AD035, NLGase, SPG46 glucosylceramidase beta 2 non-lysosomal glucosylceramidase|beta-glucocerebrosidase 2|bile acid beta-glucosidase GBA2|bile acid glucosyl transferase GBA2|cholesterol glucosyltransferase GBA2|cholesteryl-beta-glucosidase GBA2|glucosidase, beta (bile acid) 2|glucosylceramidase 2

For more info on GBA2, check out the GBA2 Infographic

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