Product Info Summary
SKU: | M00593 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GATA3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00593
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GATA3 Rabbit Monoclonal Antibody catalog # M00593. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GATA3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00593)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABBH-7
Isotype
Rabbit IgG
Standard Protein
A synthesized peptide derived from human GATA3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00593 is reactive to GATA3 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
32-42 kDa, 65 kDa, 75 kDa
Calculated molecular weight
47916 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00593 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:100
Positive Control
IHC: Human thyroid cancer tissue, Human small cell lung cancer tissue, Rat intestine tissue, Mouse pancreas tissue, Human pituitary tumor tissue, human bladder cancer tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of GATA3 expression in (1) SH-SY5Y cell lysate; (2) Jurkat cell lysate.
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Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:250 dilution.
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Immunohistochemical analysis of paraffin-embedded Human small cell lung cancer , using the Antibody at 1:1000 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat intestine, using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse pancreas, using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:250 dilution.
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Immunohistochemical analysis of paraffin-embedded human bladder cancer, using GATA3 Antibody.
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Figure 9. Western blot analysis of GATA3 using anti-GATA3 rabbit monoclonal antibody (M00593).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% milk for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA3 rabbit monoclonal antibody (M00593) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000, room temperature for 1 h. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA3 at approximately 47 kDa. The expected band size for GATA3 is at 47 kDa.
Protein Target Info & Infographic
Gene/Protein Information For GATA3 (Source: Uniprot.org, NCBI)
Gene Name
GATA3
Full Name
Trans-acting T-cell-specific transcription factor GATA-3
Weight
47916 MW
Alternative Names
131320; GATA binding protein 3; GATA3; GATA-3; GATA-binding factor 3; GATA-binding protein 3; HDRMGC5199; MGC2346; MGC5445; trans-acting T-cell-specific transcription factor GATA-3 GATA3 HDR, HDRS GATA binding protein 3 trans-acting T-cell-specific transcription factor GATA-3|GATA-binding factor 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GATA3, check out the GATA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GATA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GATA3 Rabbit Monoclonal Antibody (M00593)
Hello CJ!
M00593 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ahluwalia A,Hoa N,Ge L,Blumberg B,Levin ER.Mechanisms by Which Membrane and Nuclear ER Alpha Inhibit Adipogenesis in Cells Isolated From Female Mice. Endocrinology. 2020 Nov 1;161(11):bqaa175.doi: 10.1210/endocr/bqaa175.PMID:32976570.
Species: Mouse
M00593 usage in article: APP:CHROMATIN IMMUNOPRECIPITATION, SAMPLE:3T3-L1 CELLS, DILUTION:NA
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Customer Reviews
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1 Reviews For Anti-GATA3 Rabbit Monoclonal Antibody
0
Good GATA3 Antibody from BosterBio--Xiangrong Geng Internal medicine University of Michigan Postdoctoral Researcher
Excellent
Source: Biocompare.com
Application | Western Blot |
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Sample | HEK293T |
Detection | ECL |
"I used this antibody for western blotting and immunoprecipitation. It works well in both of them. Very good antibody."
Xiangrong Geng
Verified customer
Submitted 2020-07-30
Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
16 Customer Q&As for Anti-GATA3 Rabbit Monoclonal Antibody
Question
Our lab were satisfied with the WB result of your anti-GATA3 Rabbit Monoclonal antibody. However we have seen positive staining in t-cell nucleus. using this antibody. Is that expected? Could you tell me where is GATA3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
From what I have seen in literature, t-cell does express GATA3. Generally GATA3 expresses in nucleus. Regarding which tissues have GATA3 expression, here are a few articles citing expression in various tissues:
Cervix, and Placenta, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
T-cell, Pubmed ID: 1827068, 2017177, 2050118
Boster Scientific Support
Answered: 2020-04-29
Question
I would like using your anti-GATA3 Rabbit Monoclonal antibody for immune system development studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
E. Rodriguez
Verified customer
Asked: 2020-04-08
Answer
We appreciate your inquiry. This M00593 anti-GATA3 Rabbit Monoclonal antibody is validated on jurkat cell lysate. It is guaranteed to work for ChIP, Flow Cytometry, IF, IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-08
Question
I was wanting to use your anti-GATA3 Rabbit Monoclonal antibody for IHC for human leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
It shows on the product datasheet, M00593 anti-GATA3 Rabbit Monoclonal antibody has been tested for ChIP, Flow Cytometry, IF, IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-16
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-GATA3 Rabbit Monoclonal antibody M00593. Let me know if you need anything else.
N. Anderson
Verified customer
Asked: 2020-02-03
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-03
Question
Is there a BSA free version of anti-GATA3 Rabbit Monoclonal antibody M00593 available?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GATA3 Rabbit Monoclonal antibody M00593 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-29
Question
Our lab want to know about to test anti-GATA3 Rabbit Monoclonal antibody M00593 on human leukemic t-cell for research purposes, then I may be interested in using anti-GATA3 Rabbit Monoclonal antibody M00593 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
The products we sell, including anti-GATA3 Rabbit Monoclonal antibody M00593, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-02
Question
Our lab used your anti-GATA3 Rabbit Monoclonal antibody for IF on leukemic t-cell in a previous project. I am using human, and We want to use the antibody for Flow Cytometry next. you antibody examining leukemic t-cell as well as cervix placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for Flow Cytometry?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
I took a look at the website and datasheets of our anti-GATA3 Rabbit Monoclonal antibody and it seems that M00593 has been tested on human in both IF and Flow Cytometry. Thus M00593 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-02
Question
My question regarding product M00593, anti-GATA3 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00593 anti-GATA3 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-21
Question
I see that the anti-GATA3 Rabbit Monoclonal antibody M00593 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-29
Question
Does anti-GATA3 Rabbit Monoclonal antibody M00593 work for IHC with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
According to the expression profile of leukemic t-cell, GATA3 is highly expressed in leukemic t-cell. So, it is likely that anti-GATA3 Rabbit Monoclonal antibody M00593 will work for IHC with leukemic t-cell.
Boster Scientific Support
Answered: 2019-08-09
Question
Is this M00593 anti-GATA3 Rabbit Monoclonal antibody reactive to the isotypes of GATA3?
G. Zhao
Verified customer
Asked: 2019-08-09
Answer
The immunogen of M00593 anti-GATA3 Rabbit Monoclonal antibody is A synthesized peptide derived from human GATA3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-09
Question
Please see the WB image, lot number and protocol we used for leukemic t-cell using anti-GATA3 Rabbit Monoclonal antibody M00593. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-12
Question
We have been able to see staining in human t-cell. What should we do? Is anti-GATA3 Rabbit Monoclonal antibody supposed to stain t-cell positively?
Verified Customer
Verified customer
Asked: 2019-04-18
Answer
According to literature t-cell does express GATA3. According to Uniprot.org, GATA3 is expressed in endometrium epithelium, t-cell, cervix placenta, leukemic t-cell, among other tissues. Regarding which tissues have GATA3 expression, here are a few articles citing expression in various tissues:
Cervix, and Placenta, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
T-cell, Pubmed ID: 1827068, 2017177, 2050118
Boster Scientific Support
Answered: 2019-04-18
Question
Is a blocking peptide available for product anti-GATA3 Rabbit Monoclonal antibody (M00593)?
Verified Customer
Verified customer
Asked: 2019-01-25
Answer
We do provide the blocking peptide for product anti-GATA3 Rabbit Monoclonal antibody (M00593). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-01-25
Question
We are currently using anti-GATA3 Rabbit Monoclonal antibody M00593 for human tissue, and we are content with the ICC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-08
Answer
The anti-GATA3 Rabbit Monoclonal antibody (M00593) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-08
Question
Would M00593 anti-GATA3 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Walker
Verified customer
Asked: 2013-08-12
Answer
You can see on the product datasheet, M00593 anti-GATA3 Rabbit Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-08-12