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Product Info Summary
| SKU: | M00408 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GATA1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00408
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GATA1 Rabbit Monoclonal Antibody catalog # M00408. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GATA1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00408)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABCB-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GATA1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00408 is reactive to GATA1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
52 kDa
Calculated molecular weight
42751 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00408 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: K562 cell
IHC: mouse spleen tissue
Validation Images & Assay Conditions
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Western blot analysis of GATA1 expression in K562 cell lysate.
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Immunohistochemical analysis of paraffin-embedded mouse spleen, using GATA1 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For GATA1 (Source: Uniprot.org, NCBI)
Gene Name
GATA1
Full Name
Erythroid transcription factor
Weight
42751 MW
Alternative Names
Erythroid transcription factor;Eryf1;GATA-binding factor 1;GATA-1;GF-1;NF-E1 DNA-binding protein;GATA1;ERYF1, GF1; GATA1 ERYF1, GATA-1, GF-1, GF1, NF-E1, NFE1, XLANP, XLTDA, XLTT GATA binding protein 1 erythroid transcription factor|GATA-binding factor 1|NF-E1 DNA-binding protein|erythroid transcription factor 1|globin transcription factor 1|nuclear factor, erythroid 1|transcription factor GATA1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GATA1, check out the GATA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GATA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GATA1 Rabbit Monoclonal Antibody (M00408)
Hello CJ!
M00408 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang J,Zhuang R,Zhang X,Hu W,Cheng K,Jiang D,Shen S,Zhang Y,Ding Y,Zhang Y.CD226 is involved in megakaryocyte activation and early-stage differentiation.Mol Immunol.2019 Mar;107:123-131.doi:10.1016/j.molimm.2019.01.013.Epub 2019 Feb 6.PMID:30738249.
Species: Human,Mouse
M00408 usage in article: APP:WB, SAMPLE:DAMI CELL, DILUTION:NA
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Customer Q&As
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3 Customer Q&As for Anti-GATA1 Rabbit Monoclonal Antibody
Question
See attached the WB image, lot number and protocol we used for erythrocyte using anti-GATA1 Rabbit Monoclonal antibody M00408. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-25
Question
I see that the anti-GATA1 Rabbit Monoclonal antibody M00408 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-04-12
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-12
Question
We are currently using anti-GATA1 Rabbit Monoclonal antibody M00408 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-16
Answer
The anti-GATA1 Rabbit Monoclonal antibody (M00408) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-16
