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Product Info Summary
| SKU: | A03612-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Gastrin Antibody
SKU/Catalog Number
A03612-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Gastrin Antibody (Catalog # A03612-1). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Gastrin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03612-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Gastrin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03612-1 is reactive to GAST in Human, Mouse
Reconstitution
Calculated molecular weight
11394 MW
Background of Gastrin
Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
Marl¨¨ne Dufresne, Physiol Rev, Jul 2006; 86: 805 - 847.
T. C. Wang, Tillotson, M. Chulak, S. J. Brand, and E. V. Schmidt
D. H. Yu, Am J Physiol Gastrointest Liver Physiol, Dec 1987; 253: 793.
Masato Komori, Am J Physiol Gastrointest Liver Physiol, Dec 20
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03612-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from NIH/3T3 cells, using Gastrin antibody A03612-1.
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Western blot analysis of extracts from K562 cells (Lane 2), using Gastrin antiobdy A03612-1. The lane on the left is treated with synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using Gastrin antibody A03612-1.
Protein Target Info & Infographic
Gene/Protein Information For GAST (Source: Uniprot.org, NCBI)
Gene Name
GAST
Full Name
Gastrin
Weight
11394 MW
Superfamily
gastrin/cholecystokinin family
Alternative Names
Gastrin;Gastrin-71;Gastrin component I;Gastrin-52;G52;Big gastrin;Gastrin component II;Gastrin-34;G34;Gastrin;Gastrin component III;Gastrin-17;G17;Gastrin-14;G14;Gastrin-6;G6;GAST;GAS; GAST GAS gastrin gastrin|preprogastrin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GAST, check out the GAST Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GAST: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Gastrin Antibody (A03612-1)
Hello CJ!
A03612-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Muscovite Reverses Gastric Gland Atrophy and Intestinal Metaplasia by Promoting Cell Proliferation in Rats with Atrophic Gastritis
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Gastrin Antibody
Question
Is there a BSA free version of anti-Gastrin antibody A03612-1 available?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Gastrin antibody A03612-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-05-05
Question
I was wanting to use your anti-Gastrin antibody for IHC for human gastric mucosa on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human gastric mucosa identification?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
You can see on the product datasheet, A03612-1 anti-Gastrin antibody has been validated for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human gastric mucosa in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-30
Question
Will anti-Gastrin antibody A03612-1 work for IHC with gastric mucosa?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
According to the expression profile of gastric mucosa, GAST is highly expressed in gastric mucosa. So, it is likely that anti-Gastrin antibody A03612-1 will work for IHC with gastric mucosa.
Boster Scientific Support
Answered: 2019-08-30
Question
We are currently using anti-Gastrin antibody A03612-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
The anti-Gastrin antibody (A03612-1) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-15
Question
Would anti-Gastrin antibody A03612-1 work on zebrafish IHC with stomach?
Verified Customer
Verified customer
Asked: 2018-03-21
Answer
Our lab technicians have not validated anti-Gastrin antibody A03612-1 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Gastrin antibody A03612-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish stomach in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-21
Question
Would A03612-1 anti-Gastrin antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Jones
Verified customer
Asked: 2014-04-18
Answer
It shows on the product datasheet, A03612-1 anti-Gastrin antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-04-18
