Anti-GAS7 Antibody

Product Name

Anti-GAS7 Antibody

View all GAS7 Antibodies

SKU/Catalog Number

A06548

Size

100ul

Form

Liquid

Description

Boster Bio Anti-GAS7 Antibody catalog # A06548. Tested in WB,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GAS7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06548)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human LS14A protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A06548 is reactive to GAS7 in Human, Mouse, Rat

Applications

A06548 is guaranteed for IF, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

54371 MW

Background of GAS7

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Marino G., J. Biol. Chem. 278:3671-3678(2003).
Kabeya Y., J. Cell Sci. 117:2805-2812(2004).
Ross M.T., Nature 434:325-337(2005

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:2000
ICC/IF: 1:50-1:200

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blotting validation for Anti-GAS7 Antibody A06548

Western blot analysis of extracts of various cell lines

Gene/Protein Information For GAS7 (Source: Uniprot.org, NCBI)

Alternative Names

GAS-7; growth arrest-specific 7; KIAA0394growth arrest-specific protein 7; MGC1348; MLL/GAS7 fusion protein; MLL/GAS7 GAS7 growth arrest specific 7 growth arrest-specific protein 7

For more info on GAS7, check out the GAS7 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GAS7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the GAS7 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis