Anti-Cyclin E2 CCNE2 Antibody

Product Name

Anti-Cyclin E2 CCNE2 Antibody

View all Cyclin E2 Antibodies

SKU/Catalog Number

A04536

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Cyclin E2 CCNE2 Antibody (Catalog # A04536). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Cyclin E2 CCNE2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04536)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids at the carboxyl terminus of the Cyclin E2 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04536 is reactive to CCNE2 in Human, Mouse

Applications

A04536 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

46757 MW

Background of Cyclin E2

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Cyclin E was first identified by its ability to rescue growth of yeast deficient in G1 Cyclins, indicating a role in G1 or G1/S transitions. Over-expression of Cyclin E has been observed in a variety of human tumors. Multiple isoforms of Cyclin E are expressed in tumors but not in normal tissues, suggesting a post-transcriptional regulation of Cyclin E.  Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27Kip1 and p21Cip1. The catalytic activity associated with Cyclin E2 complexes is cell cycle regulated and peaks at the G1/S transition.  Unlike Cyclin E1, which is expressed in most proliferating normal and tumor cells, Cyclin E2 levels were low to undetectable in non-transformed cells and increased significantly in tumor-derived cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:85,000
IP: 1:100
WB: 1:200 - 1:2,000

Validation Images & Assay Conditions

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Western blot analysis of Cyclin E2 expression in Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2). Cyclin E2 was detected using rabbit anti-Cyclin E2 Antigen Affinity purified polyclonal antibody (Catalog # A04536). Personal Communication, Philipp Kaldis, CCR-NCI, Frederick, MD.

Gene/Protein Information For CCNE2 (Source: Uniprot.org, NCBI)

Alternative Names

CCNE2; CYCE2; Cyclin E2; G1/S-specific cyclin E2; G1/S-specific cyclin-E2 CCNE2 CYCE2 cyclin E2 G1/S-specific cyclin-E2

For more info on CCNE2, check out the CCNE2 Infographic

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In this infographic, you will see the following information for CCNE2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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