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Product Info Summary
| SKU: | M04618 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-GARS Rabbit Monoclonal Antibody
SKU/Catalog Number
M04618
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GARS Rabbit Monoclonal Antibody catalog # M04618. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GARS Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04618)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FEA-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GARS
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04618 is reactive to GARS in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
130 kDa
Calculated molecular weight
83166 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04618 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
Positive Control
WB: Jurkat cell, 293T cell
IHC: human kidney tissue
Validation Images & Assay Conditions
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Western blot analysis of GARS expression in (1) Jurkat cell lysate; (2) 293T cell lysate.
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Immunohistochemical analysis of paraffin-embedded human kidney, using GARS Antibody.
Protein Target Info & Infographic
Gene/Protein Information For GARS (Source: Uniprot.org, NCBI)
Gene Name
GARS
Full Name
Glycine--tRNA ligase
Weight
83166 MW
Superfamily
class-II aminoacyl-tRNA synthetase family
Alternative Names
Glycine--tRNA ligase;3.6.1.17 ;6.1.1.14 ;Diadenosine tetraphosphate synthetase;AP-4-A synthetase;Glycyl-tRNA synthetase;GlyRS;GARS; GARS1 CMT2D, DSMAV, GARS, GlyRS, HMN5, HMN5A, SMAD1, SMAJI glycyl-tRNA synthetase 1 glycine--tRNA ligase|AP-4-A synthetase|Charcot-Marie-Tooth neuropathy 2D|Charcot-Marie-Tooth neuropathy, neuronal type, D|ap4A synthetase|diadenosine tetraphosphate synthetase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GARS, check out the GARS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GARS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GARS Rabbit Monoclonal Antibody (M04618)
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Customer Q&As
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7 Customer Q&As for Anti-GARS Rabbit Monoclonal Antibody
Question
We are currently using anti-GARS Rabbit Monoclonal antibody M04618 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
The anti-GARS Rabbit Monoclonal antibody (M04618) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-06
Question
My lab would like to test anti-GARS Rabbit Monoclonal antibody M04618 on human secondary oocyte for research purposes, then I may be interested in using anti-GARS Rabbit Monoclonal antibody M04618 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
The products we sell, including anti-GARS Rabbit Monoclonal antibody M04618, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-19
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for secondary oocyte using anti-GARS Rabbit Monoclonal antibody M04618. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-13
Question
Will M04618 anti-GARS Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Zhang
Verified customer
Asked: 2018-09-17
Answer
As indicated on the product datasheet, M04618 anti-GARS Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-17
Question
My question regarding product M04618, anti-GARS Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-07-25
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M04618 anti-GARS Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-07-25
Question
Do you have a BSA free version of anti-GARS Rabbit Monoclonal antibody M04618 available?
Verified Customer
Verified customer
Asked: 2017-07-12
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-GARS Rabbit Monoclonal antibody M04618 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-12
Question
Does anti-GARS Rabbit Monoclonal antibody M04618 work on monkey WB with liver?
W. Collins
Verified customer
Asked: 2015-03-19
Answer
Our lab technicians have not validated anti-GARS Rabbit Monoclonal antibody M04618 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-GARS Rabbit Monoclonal antibody M04618 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-03-19
