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Product Info Summary
| SKU: | M05934 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-gamma Sarcoglycan Rabbit Monoclonal Antibody
View all gamma Sarcoglycan Antibodies
SKU/Catalog Number
M05934
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-gamma Sarcoglycan Rabbit Monoclonal Antibody catalog # M05934. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-gamma Sarcoglycan Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05934)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AbS34
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human gamma Sarcoglycan
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05934 is reactive to SGCG in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
74,76 kDa
Calculated molecular weight
32379 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05934 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Positive Control
IHC: mouse skeletal muscle tissue
Validation Images & Assay Conditions
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Western blot analysis of gamma Sarcoglycan expression in Human skeletal muscle lysate.
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Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-gamma Sarcoglycan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For SGCG (Source: Uniprot.org, NCBI)
Gene Name
SGCG
Full Name
Gamma-sarcoglycan
Weight
32379 MW
Superfamily
sarcoglycan beta/delta/gamma/zeta family
Alternative Names
Gamma-sarcoglycan;Gamma-SG;35 kDa dystrophin-associated glycoprotein;35DAG;SGCG; SGCG 35DAG, A4, DAGA4, DMDA, DMDA1, LGMD2C, LGMDR5, MAM, SCARMD2, SCG3, gamma-SG sarcoglycan gamma gamma-sarcoglycan|35 kDa dystrophin-associated glycoprotein|35kD dystrophin-associated glycoprotein|sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SGCG, check out the SGCG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SGCG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-gamma Sarcoglycan Rabbit Monoclonal Antibody (M05934)
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No publications found for M05934
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Customer Q&As
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7 Customer Q&As for Anti-gamma Sarcoglycan Rabbit Monoclonal Antibody
Question
I was wanting to use to test anti-gamma Sarcoglycan Rabbit Monoclonal antibody M05934 on human vastus lateralis for research purposes, then I may be interested in using anti-gamma Sarcoglycan Rabbit Monoclonal antibody M05934 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
The products we sell, including anti-gamma Sarcoglycan Rabbit Monoclonal antibody M05934, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-11
Question
I see that the anti-gamma Sarcoglycan Rabbit Monoclonal antibody M05934 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-23
Question
Is this M05934 anti-gamma Sarcoglycan Rabbit Monoclonal antibody reactive to the isotypes of SGCG?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
The immunogen of M05934 anti-gamma Sarcoglycan Rabbit Monoclonal antibody is A synthesized peptide derived from human gamma Sarcoglycan. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-31
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for vastus lateralis using anti-gamma Sarcoglycan Rabbit Monoclonal antibody M05934. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-08-24
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-24
Question
Does anti-gamma Sarcoglycan Rabbit Monoclonal antibody M05934 work for IP with vastus lateralis?
Verified Customer
Verified customer
Asked: 2018-02-08
Answer
According to the expression profile of vastus lateralis, SGCG is highly expressed in vastus lateralis. So, it is likely that anti-gamma Sarcoglycan Rabbit Monoclonal antibody M05934 will work for IP with vastus lateralis.
Boster Scientific Support
Answered: 2018-02-08
Question
We are currently using anti-gamma Sarcoglycan Rabbit Monoclonal antibody M05934 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-17
Answer
The anti-gamma Sarcoglycan Rabbit Monoclonal antibody (M05934) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-17
Question
Is a blocking peptide available for product anti-gamma Sarcoglycan Rabbit Monoclonal antibody (M05934)?
R. Krishna
Verified customer
Asked: 2015-10-12
Answer
We do provide the blocking peptide for product anti-gamma Sarcoglycan Rabbit Monoclonal antibody (M05934). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-10-12
