Product Info Summary
SKU: | PA2122 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-C-C chemokine receptor type 9 CCR9 Antibody Picoband®
SKU/Catalog Number
PA2122
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-C-C chemokine receptor type 9 CCR9 Antibody catalog # PA2122. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-C-C chemokine receptor type 9 CCR9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2122)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CCR9.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2122 is reactive to CCR9 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
42 kDa
Calculated molecular weight
42016 MW
Background of CCR9
C-C chemokine receptor type 9, also called GPR-9-6 or CDw199, is a protein that in humans is encoded by the CCR9 gene. The protein encoded by this gene is a member of the beta chemoking receptor family. By radiation hybrid analysis and organization of BAC contigs by FISH on combed genomic DNA, the CCR9 gene within the CCR cluster was localized at 3p21.3. It is the receptor for chemokine SCYA25/TECK. This gene subsequently transduces a signal by increasing the intracellular calcium ions level. It is an alternative coreceptor with CD4 for HIV-1 infection. This gene may play a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2122 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: JURKAT Whole Cell, HELA Whole Cell, SMMC Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-CCR9 antibody, PA2122, Western blotting
All lanes: Anti CCR9 (PA2122) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 42KD
Observed bind size: 42KD
Protein Target Info & Infographic
Gene/Protein Information For CCR9 (Source: Uniprot.org, NCBI)
Gene Name
CCR9
Full Name
C-C chemokine receptor type 9
Weight
42016 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
C-C chemokine receptor type 9;C-C CKR-9;CC-CKR-9;CCR-9;G-protein coupled receptor 28;GPR-9-6;CDw199;CCR9;GPR28; CCR9 CC-CKR-9, CDw199, GPR-9-6, GPR28 C-C motif chemokine receptor 9 C-C chemokine receptor type 9|G protein-coupled receptor 28|chemokine (C-C motif) receptor 9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCR9, check out the CCR9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCR9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-C-C chemokine receptor type 9 CCR9 Antibody Picoband® (PA2122)
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No publications found for PA2122
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Customer Q&As
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6 Customer Q&As for Anti-C-C chemokine receptor type 9 CCR9 Antibody Picoband®
Question
Here is the WB image, lot number and protocol we used for thymus using anti-CCR9 antibody PA2122. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-22
Question
We are currently using anti-CCR9 antibody PA2122 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
The anti-CCR9 antibody (PA2122) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-30
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thymus using anti-CCR9 antibody PA2122. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-02
Question
We are interested in to test anti-CCR9 antibody PA2122 on human thymus for research purposes, then I may be interested in using anti-CCR9 antibody PA2122 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
The products we sell, including anti-CCR9 antibody PA2122, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-08
Question
Is this PA2122 anti-CCR9 antibody reactive to the isotypes of CCR9?
Verified Customer
Verified customer
Asked: 2017-07-26
Answer
The immunogen of PA2122 anti-CCR9 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human CCR9(1-17aa MTPTDFTSPIPNMADDY). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-26
Question
Is a blocking peptide available for product anti-CCR9 antibody (PA2122)?
A. Collins
Verified customer
Asked: 2016-11-24
Answer
We do provide the blocking peptide for product anti-CCR9 antibody (PA2122). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-11-24