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Product Info Summary
| SKU: | A09635 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-GALNT10 Antibody
View all Polypeptide GalNac Transferase 10/GALNT10 Antibodies
SKU/Catalog Number
A09635
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-GALNT10 Antibody (Catalog # A09635). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
GALNT10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-GALNT10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09635)
Host
Rabbit
Contents
GALNT10 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
GALNT10 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human GALNT10. The immunogen is located within amino acids 530 - 580 of GALNT10.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
VEZT antibody is human, mouse and rat reactive. Multiple isoforms are known to exist.
Reactive Species
A09635 is reactive to GALNT10 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
68992 MW
Background of Polypeptide GalNac Transferase 10/GALNT10
Protein glycosylation is an important biological process that is carried out by a large family of glycosyltransferases that catalyze the synthesis of oligosaccharides and glycoconjugates. Polypeptide GalNAc transferases initiate the synthesis of mucin-type oligosaccharides by transferring GalNAc from UDP-GalNAc to the hydroxyl group of either a serine or threonine residue on the polypeptide acceptor. Polypeptide galactoaminyltransferase 10 (GALNT10) belongs to the polypeptide N-acetylgalactosaminyl-transferase (pp-GalNAc-T) protein family. Following expression in insect cells, recombinant GALNT10 showed significant GalNAcT activity toward mucin-derived peptides, and it utilized both non-glycosylated and glycosylated peptide substrates. GALNT10 mRNA is highly expressed in several distinct hypothalamic, thalamic, and amygdaloid nuclei in mouse brain. At least four isoforms of GALNT10 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09635 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
GALNT10 antibody can be used for detection of GALNT10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of GALNT10 in SK-N-SH cell lysate with GALNT10 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of GALNT10 in human brain tissue with GALNT10 antibody at 2.5 μg/mL.
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Immunofluorescence of GALNT10 in Human Brain cells with GALNT10 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For GALNT10 (Source: Uniprot.org, NCBI)
Gene Name
GALNT10
Full Name
Polypeptide N-acetylgalactosaminyltransferase 10
Weight
68992 MW
Superfamily
glycosyltransferase 2 family
Alternative Names
GALNACT10, PPGALNACT10, PPGANTASE10, Polypeptide N-acetylgalactosaminyltransferase 10, Polypeptide GalNAc transferase 10, GalNAc-T10 GALNT10 GALNACT10, PPGALNACT10, PPGANTASE10 polypeptide N-acetylgalactosaminyltransferase 10 polypeptide N-acetylgalactosaminyltransferase 10|GalNAc transferase 10|UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 10|UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 10 (GalNAc-T10)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GALNT10, check out the GALNT10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GALNT10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GALNT10 Antibody (A09635)
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