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Product Info Summary
| SKU: | A01135-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Galactosidase alpha/Gla Antibody Picoband®
View all alpha-Galactosidase A/GLA Antibodies
SKU/Catalog Number
A01135-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Galactosidase alpha/Gla Antibody Picoband® catalog # A01135-2. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Galactosidase alpha/Gla Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01135-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Galactosidase alpha/Gla.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01135-2 is reactive to GLA in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
49 kDa
Calculated molecular weight
47643 MW
Background of alpha-Galactosidase A/GLA
Alpha-galactosidase is a glycoside hydrolase enzyme that encoded by the GLA gene. This gene is a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01135-2 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Positive Control
WB: human HELA whole cell, human MCF-7 whole cell, human HEK293 whole cell
IHC: human mammary cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Galactosidase alpha/Gla using anti-Galactosidase alpha/Gla antibody (A01135-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HEK293 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galactosidase alpha/Gla antigen affinity purified polyclonal antibody (Catalog # A01135-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galactosidase alpha/Gla at approximately 49KD. The expected band size for Galactosidase alpha/Gla is at 49KD.
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Figure 2. IHC analysis of Galactosidase alpha/Gla using anti-Galactosidase alpha/Gla antibody (A01135-2).
Galactosidase alpha/Gla was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Galactosidase alpha/Gla Antibody (A01135-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For GLA (Source: Uniprot.org, NCBI)
Gene Name
GLA
Full Name
Alpha-galactosidase A
Weight
47643 MW
Superfamily
glycosyl hydrolase 27 family
Alternative Names
Alpha-galactosidase A;3.2.1.22 ;Alpha-D-galactosidase A;Alpha-D-galactoside galactohydrolase;Melibiase;Gla;Ags; GLA GALA galactosidase alpha alpha-galactosidase A|agalsidase alfa|alpha-D-galactosidase A|alpha-D-galactoside galactohydrolase 1|alpha-gal A|galactosylgalactosylglucosylceramidase GLA|melibiase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GLA, check out the GLA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GLA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Galactosidase alpha/Gla Antibody Picoband® (A01135-2)
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