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Product Info Summary
| SKU: | PA1403 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-GAD65/GAD2 Antibody Picoband®
View all GAD2/GAD65 Antibodies
SKU/Catalog Number
PA1403
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GAD65/GAD2 Antibody catalog # PA1403. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GAD65/GAD2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1403)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human GAD65, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1403 is reactive to GAD2 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
65411 MW
Background of GAD2/GAD65
Glutamate decarboxylase 2, also known as GAD65, is an enzyme that in humans is encoded by the GAD2 gene. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1403 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Rat, By Heat
Positive Control
WB: rat brain tissue, mouse brain tissue
IHC: rat brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GAD65/GAD2 using anti-GAD65/GAD2 antibody (PA1403).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAD65/GAD2 antigen affinity purified polyclonal antibody (Catalog # PA1403) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAD65/GAD2 at approximately 65 kDa. The expected band size for GAD65/GAD2 is at 65 kDa.
Click image to see more details
Figure 2. IHC analysis of GAD65/GAD2 using anti-GAD65/GAD2 antibody (PA1403).
GAD65/GAD2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GAD65/GAD2 Antibody (PA1403) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For GAD2 (Source: Uniprot.org, NCBI)
Gene Name
GAD2
Full Name
Glutamate decarboxylase 2
Weight
65411 MW
Superfamily
group II decarboxylase family
Alternative Names
Glutamate decarboxylase 2;4.1.1.15;65 kDa glutamic acid decarboxylase;GAD-65;Glutamate decarboxylase 65 kDa isoform;GAD2;GAD65; GAD2 GAD65 glutamate decarboxylase 2 glutamate decarboxylase 2|65 kDa glutamic acid decarboxylase|GAD-65|Glutamate decarboxylase-2 (pancreas)|glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GAD2, check out the GAD2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GAD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GAD65/GAD2 Antibody Picoband® (PA1403)
Hello CJ!
PA1403 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Acrylamide neurotoxicity on the cerebrum of weaning rats
Shi J, Ma Y, Zheng M, Ruan Z, Liu J, Tian S, Zhang D, He X, Li G. Toxicol Ind Health. 2012 Feb;28(1):10-20. Doi: 10.1177/0748233711401264. Epub 2011 Mar 28. Effect Of Sub-Acute Exposure To Acrylamide On Gabaergic Neurons And Astrocytes In Weaning ...
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-GAD65/GAD2 Antibody Picoband®
Question
Is this PA1403 anti-GAD65/GAD2 antibody reactive to the isotypes of GAD2?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
The immunogen of PA1403 anti-GAD65/GAD2 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human GAD65(21-37aa ENPGTARAWCQVAQKFT), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-25
Question
We are currently using anti-GAD65/GAD2 antibody PA1403 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
The anti-GAD65/GAD2 antibody (PA1403) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-30
Question
Is a blocking peptide available for product anti-GAD65/GAD2 antibody (PA1403)?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
We do provide the blocking peptide for product anti-GAD65/GAD2 antibody (PA1403). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-02
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreatic islet using anti-GAD65/GAD2 antibody PA1403. Let me know if you need anything else.
E. Edwards
Verified customer
Asked: 2015-12-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-12-04
Question
Can you help my question with product PA1403, anti-GAD65/GAD2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Anderson
Verified customer
Asked: 2014-01-23
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1403 anti-GAD65/GAD2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-01-23
Question
I was wanting to use your anti-GAD65/GAD2 antibody for WB for human pancreatic islet on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pancreatic islet identification?
O. Dhar
Verified customer
Asked: 2013-10-04
Answer
You can see on the product datasheet, PA1403 anti-GAD65/GAD2 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human pancreatic islet in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-10-04
