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Product Info Summary
| SKU: | A03996-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-GABRA2 Antibody (C-term)
View all GABA-AR alpha 2 Antibodies
SKU/Catalog Number
A03996-1
Size
80 µl
Description
Boster Bio Anti-GABRA2 Antibody (C-term) (Catalog # A03996-1). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-GABRA2 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03996-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This GABRA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 378-406 amino acids from the C-terminal region of human GABRA2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03996-1 is reactive to GABRA2 in Human
Reconstitution
Calculated molecular weight
51326 Da
Background of GABA-AR alpha 2
GABRA2 is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03996-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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GABRA2 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GABRA2 antibody detected the GABRA2 protein (arrow).
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GABRA2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GABRA2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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GABRA2 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For GABRA2 (Source: Uniprot.org, NCBI)
Gene Name
GABRA2
Full Name
Gamma-aminobutyric acid receptor subunit alpha-2
Weight
51326 Da
Superfamily
ligand-gated ion channel (TC 1.A.9) family
Alternative Names
Gamma-aminobutyric acid receptor subunit alpha-2, GABA(A) receptor subunit alpha-2, GABRA2 GABRA2 DEE78, EIEE78 gamma-aminobutyric acid type A receptor subunit alpha2 gamma-aminobutyric acid receptor subunit alpha-2|GABA(A) receptor subunit alpha-2|gamma-aminobutyric acid type A receptor alpha2 subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GABRA2, check out the GABRA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GABRA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GABRA2 Antibody (C-term) (A03996-1)
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