Product Info Summary
SKU: | M01907 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GABARAP Monoclonal Antibody
SKU/Catalog Number
M01907
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GABARAP Monoclonal Antibody catalog # M01907. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GABARAP Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01907)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACHE-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GABARAP GABARAP is cleaved at its carboxyl terminus, which leads to conjugation by either of the phospholipids phosphatidylethanolamine or phosphatidylserine. This processing converts GABARAP from a type I to a type II membrane bound form involved in autophagosome biogenesis. Processing of GABARAP involves cleavage by Atg4 family members followed by conjugation by the E1 and E2 like enzymes Atg7 and Atg3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01907 is reactive to GABARAP in Human, Mouse, Rat
Applications
M01907 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
18 kDa
Calculated molecular weight
43296 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:500
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of GABARAP using anti-GABARAP antibody (M01907).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human HUH7 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GABARAP antigen affinity purified monoclonal antibody (Catalog # M01907) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GABARAP at approximately 18 kDa. The expected band size for GABARAP is at 14 kDa.
Protein Target Info & Infographic
Gene/Protein Information For GABARAP (Source: Uniprot.org, NCBI)
Gene Name
GABARAP
Full Name
Gamma-aminobutyric acid receptor-associated protein
Weight
43296 MW
Superfamily
ATG8 family
Alternative Names
Apg8p1; ATG8A; FLC3B; FLJ25768; GABA(A) receptor-associated proteinMGC120154; GABARAP; gamma-aminobutyric acid receptor-associated protein; MM46; MM46MGC120155 GABARAP ATG8A-a, MM46, GABARAP GABA type A receptor-associated protein gamma-aminobutyric acid receptor-associated protein|GABA(A) receptor-associated protein|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GABARAP, check out the GABARAP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GABARAP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GABARAP Monoclonal Antibody (M01907)
Hello CJ!
M01907 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhan LH,Dong YJ,Yang K,Lei SS,Li B,Teng X,Zhou C,Luo R,Yu QX,Jin HY,Lv GY,Chen SH.Soporific Effect of Modified Suanzaoren Decoction and Its Effects on the Expression of CCK-8 and Orexin-A.Evid Based Complement Alternat Med.2020 Jun 16;2020:6984087.doi:10.
Species: Mouse,Rat
M01907 usage in article: APP:WB, SAMPLE:BRAIN AND GASTRIC TISSUE, DILUTION:NA
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Customer Q&As
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3 Customer Q&As for Anti-GABARAP Monoclonal Antibody
Question
I see that the anti-GABARAP Monoclonal antibody M01907 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-20
Question
Does M01907 anti-GABARAP Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Johnson
Verified customer
Asked: 2017-09-21
Answer
As indicated on the product datasheet, M01907 anti-GABARAP Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-09-21
Question
We are currently using anti-GABARAP Monoclonal antibody M01907 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
J. Collins
Verified customer
Asked: 2016-08-11
Answer
The anti-GABARAP Monoclonal antibody (M01907) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-08-11