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Product Info Summary
| SKU: | PA1578 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-GABA A Receptor alpha 1/GABRA1 Antibody Picoband®
View all GABA-AR alpha 1 Antibodies
SKU/Catalog Number
PA1578
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GABA A Receptor alpha 1/GABRA1 Antibody catalog # PA1578. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GABA A Receptor alpha 1/GABRA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1578)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human GABA A Receptor alpha 1, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1578 is reactive to GABRA1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
52 kDa
Calculated molecular weight
51802 MW
Background of GABA-AR alpha 1
GABRA1, Gamma-aminobutyric acid receptor subunit alpha-1, is a protein that in humans is encoded by the GABRA1 gene. The 1,055-bp GABRA1 clone contained an open reading frame and 260 nucleotides in the 5-prime noncoding region. The 351-amino acid sequence shows 97% homology with its bovine counterpart. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1578 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Brain Tissue, Rat Brain Tissue,, human Hela whole cell, human U-87MG whole cell
IHC: Rat Brain Tissue
Validation Images & Assay Conditions
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Anti-GABA A Receptor alpha 1 antibody, PA1578, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
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Anti-GABA A Receptor alpha 1 antibody, PA1578, IHC(P)
IHC(P): Rat Brain Tissue
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Figure 3. Western blot analysis of GABRA1 using anti-GABRA1 antibody (PA1578).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GABRA1 antigen affinity purified polyclonal antibody (Catalog # PA1578) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GABRA1 at approximately 52KD. The expected band size for GABRA1 is at 52KD.
Protein Target Info & Infographic
Gene/Protein Information For GABRA1 (Source: Uniprot.org, NCBI)
Gene Name
GABRA1
Full Name
Gamma-aminobutyric acid receptor subunit alpha-1
Weight
51802 MW
Superfamily
ligand-gated ion channel (TC 1.A.9) family
Alternative Names
Gamma-aminobutyric acid receptor subunit alpha-1;GABA (A) receptor subunit alpha-1;GABRA1; GABRA1 DEE19, ECA4, EIEE19, EJM, EJM5 gamma-aminobutyric acid type A receptor subunit alpha1 gamma-aminobutyric acid receptor subunit alpha-1|GABA(A) receptor subunit alpha-1|GABA(A) receptor, alpha 1|gamma-aminobutyric acid (GABA) A receptor, alpha 1|gamma-aminobutyric acid type A receptor alpha1 subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GABRA1, check out the GABRA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GABRA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GABA A Receptor alpha 1/GABRA1 Antibody Picoband® (PA1578)
Hello CJ!
PA1578 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Role of microRNA-4458 in patients with non-small-cell lung cancer
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Customer Q&As
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1 Customer Q&As for Anti-GABA A Receptor alpha 1/GABRA1 Antibody Picoband®
Question
We are currently using anti-GABA A Receptor alpha 1/GABRA1 antibody PA1578 for human tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
The anti-GABA A Receptor alpha 1/GABRA1 antibody (PA1578) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-13
