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Product Info Summary
| SKU: | A04598-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-G alpha t1 Antibody
View all Transducin alpha Antibodies
SKU/Catalog Number
A04598-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-G alpha t1 Antibody catalog # A04598-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-G alpha t1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04598-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human C4B
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04598-1 is reactive to GNAT1 in Human, Mouse, Rat
Applications
A04598-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
40041 MW
Background of Transducin alpha
May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production.
Pennica D., Swanson T.A., Welsh J.W. J.Proc. Natl. Acad. Sci. U.S.A. 95:14717-14722(1998)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using Galpha t1 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For GNAT1 (Source: Uniprot.org, NCBI)
Gene Name
GNAT1
Full Name
Guanine nucleotide-binding protein G(t) subunit alpha-1
Weight
40041 MW
Superfamily
G-alpha family
Alternative Names
CSNBAD3; GBT1; GNATR; guanine nucleotide binding protein (G protein), alpha transducing activitypolypeptide 1; guanine nucleotide-binding protein G(t) subunit alpha-1; guanine nucleotide-binding protein G(T), alpha-1 subunit; rod-type transducin alpha subunit; Transducin alpha-1 chain; transducin, rod-specific GNAT1 CSNB1G, CSNBAD3, GBT1, GNATR G protein subunit alpha transducin 1 guanine nucleotide-binding protein G(t) subunit alpha-1|guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 1|guanine nucleotide-binding protein G(T), alpha-1 subunit|rod-type transducin alpha subunit|transducin alpha-1 chain|transducin, rod-specific
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GNAT1, check out the GNAT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GNAT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-G alpha t1 Antibody (A04598-1)
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No publications found for A04598-1
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Customer Reviews
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Customer Q&As
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12 Customer Q&As for Anti-G alpha t1 Antibody
Question
We are currently using anti-G alpha t1 antibody A04598-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
The anti-G alpha t1 antibody (A04598-1) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-07
Question
I see that the anti-G alpha t1 antibody A04598-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-13
Question
Is a blocking peptide available for product anti-G alpha t1 antibody (A04598-1)?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
We do provide the blocking peptide for product anti-G alpha t1 antibody (A04598-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-20
Question
Would anti-G alpha t1 antibody A04598-1 work for WB with pigmented layer of retina?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
According to the expression profile of pigmented layer of retina, GNAT1 is highly expressed in pigmented layer of retina. So, it is likely that anti-G alpha t1 antibody A04598-1 will work for WB with pigmented layer of retina.
Boster Scientific Support
Answered: 2019-05-20
Question
I was wanting to use your anti-G alpha t1 antibody for WB for rat pigmented layer of retina on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat pigmented layer of retina identification?
R. Gonzalez
Verified customer
Asked: 2019-04-29
Answer
You can see on the product datasheet, A04598-1 anti-G alpha t1 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat pigmented layer of retina in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-29
Question
I have a question about product A04598-1, anti-G alpha t1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-04-16
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04598-1 anti-G alpha t1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-04-16
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pigmented layer of retina using anti-G alpha t1 antibody A04598-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-02-26
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-26
Question
Will A04598-1 anti-G alpha t1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-12-11
Answer
It shows on the product datasheet, A04598-1 anti-G alpha t1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-12-11
Question
Is there a BSA free version of anti-G alpha t1 antibody A04598-1 available?
Verified Customer
Verified customer
Asked: 2018-05-02
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-G alpha t1 antibody A04598-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-02
Question
Would anti-G alpha t1 antibody A04598-1 work on horse WB with pigmented layer of retina?
C. Wu
Verified customer
Asked: 2018-01-12
Answer
Our lab technicians have not validated anti-G alpha t1 antibody A04598-1 on horse. You can run a BLAST between horse and the immunogen sequence of anti-G alpha t1 antibody A04598-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse pigmented layer of retina in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-12
Question
Here is the WB image, lot number and protocol we used for pigmented layer of retina using anti-G alpha t1 antibody A04598-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-11-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-01
Question
I was wanting to use to test anti-G alpha t1 antibody A04598-1 on rat pigmented layer of retina for research purposes, then I may be interested in using anti-G alpha t1 antibody A04598-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Yang
Verified customer
Asked: 2014-04-22
Answer
The products we sell, including anti-G alpha t1 antibody A04598-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-04-22
