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Product Info Summary
| SKU: | M00219 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-FTO Monoclonal Antibody
SKU/Catalog Number
M00219
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-FTO Monoclonal Antibody catalog # M00219. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FTO Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00219)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEAA-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FTO Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00219 is reactive to FTO in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
58 kDa
Calculated molecular weight
14460 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00219 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: human 293T whole cell, human Hela whole cell, human SiHa whole cell, human Daudi whole cell, human HepG2 whole cell, human T-47D whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FTO using anti-FTO antibody (M00219).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: human Daudi whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human T-47D whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FTO antigen affinity purified monoclonal antibody (Catalog # M00219) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FTO at approximately 58 kDa. The expected band size for FTO is at 58 kDa.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
Protein Target Info & Infographic
Gene/Protein Information For FTO (Source: Uniprot.org, NCBI)
Gene Name
FTO
Full Name
Alpha-ketoglutarate-dependent dioxygenase FTO
Weight
14460 MW
Superfamily
fto family
Alternative Names
Alpha-synuclein;Non-A beta component of AD amyloid;Non-A4 component of amyloid precursor;NACP;SNCA;NACP, PARK1; Fto|AW743446, mKIAA1752|fat mass and obesity associated|alpha-ketoglutarate-dependent dioxygenase FTO|U6 small nuclear RNA (2-O-methyladenosine-N(6)-)-demethylase FTO|U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO|fat mass and obesity-associated protein|m6A(m)-demethylase FTO|mRNA (2-O-methyladenosine-N(6)-)-demethylase FTO|mRNA N(6)-methyladenosine demethylase FTO|protein fatso|tRNA N1-methyl adenine demethylase FTO
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FTO, check out the FTO Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FTO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FTO Monoclonal Antibody (M00219)
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No publications found for M00219
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Customer Q&As
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1 Customer Q&As for Anti-FTO Monoclonal Antibody
Question
We are currently using anti-FTO Monoclonal antibody M00219 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
The anti-FTO Monoclonal antibody (M00219) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-14
