Anti-FTO Antibody

Product Name

Anti-FTO Antibody

View all Fto Antibodies

SKU/Catalog Number

A00219

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-FTO Antibody (Catalog # A00219). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

Storage & Handling

FTO antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-FTO Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00219)

Host

Rabbit

Contents

FTO Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

FTO antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human FTO. The immunogen is located within the first 50 amino acids of FTO.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least two isoforms of IL-15 are known to exist.

Reactive Species

A00219 is reactive to FTO in Human

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

58282 MW

Background of Fto

Rising obesity rates are rapidly becoming a growing health concern in the developing world. The fat mass and obesity associated gene (FTO) is the first gene discovered to contribute to common forms of human obesity. FTO is a member of the non-heme dioxygenase superfamily, encoding a 2-oxoglutarate-dependent nucleic acid demethylase whose mRNA is widely expressed, especially in neurons of feeding-related nuclei of the brain. FTO mRNA in the arcuate nucleus in mice is up-regulated by feeding and down-regulated during fasting, although the opposite pattern has been observed in rats. At least four isoforms of FTO are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00219 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

FTO antibody can be used for detection of FTO by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For FTO (Source: Uniprot.org, NCBI)

Alternative Names

ALKBH9, KIAA1752, Alpha-ketoglutarate-dependent dioxygenase FTO, Fat mass and obesity-associated protein Fto|AW743446, mKIAA1752|fat mass and obesity associated|alpha-ketoglutarate-dependent dioxygenase FTO|U6 small nuclear RNA (2-O-methyladenosine-N(6)-)-demethylase FTO|U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO|fat mass and obesity-associated protein|m6A(m)-demethylase FTO|mRNA (2-O-methyladenosine-N(6)-)-demethylase FTO|mRNA N(6)-methyladenosine demethylase FTO|protein fatso|tRNA N1-methyl adenine demethylase FTO

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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