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Product Info Summary
| SKU: | PB9106 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-FSH beta/FSHB Antibody Picoband®
SKU/Catalog Number
PB9106
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FSH beta/FSHB Antibody Picoband® catalog # PB9106. Tested in IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FSH beta/FSHB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9106)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human FSH beta recombinant protein (Position: N19-E129). Human FSH beta shares 90% and 89% amino acid (aa) sequences identity with mouse and rat FSH beta, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9106 is reactive to FSHB in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
14700 MW
Background of FSH beta
FSHB, also known as Follitropin subunit beta, is a protein that in humans is encoded by the FSHB gene, and this gene is mapped to 11p14.1. FSHB enables ovarian folliculogenesis to the antral follicle stage and is essential for Sertoli cell proliferation and maintenance of sperm quality in the testis. It can stimulate development of follicle and spermatogenesis in the reproductive organs. This gene encodes the beta subunit of follicle-stimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9106 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
IHC: mouse brain tissue, human mammry cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FSH beta using anti-FSH beta antibody (PB9106).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours
Lane 1: recombinant human FSH beta protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSH beta antigen affinity purified polyclonal antibody (Catalog # PB9106) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FSH beta at approximately 36 kDa. The expected band size for FSH beta is at 36 kDa.
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Figure 2. IHC analysis of FSH beta using anti-FSH beta antibody (PB9106).
FSH beta was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FSH beta Antibody (PB9106) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of FSH beta using anti-FSH beta antibody (PB9106).
FSH beta was detected in a paraffin-embedded section of human mammry cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FSH beta Antibody (PB9106) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For FSHB (Source: Uniprot.org, NCBI)
Gene Name
FSHB
Full Name
Follitropin subunit beta
Weight
14700 MW
Superfamily
glycoprotein hormones subunit beta family
Alternative Names
Follitropin subunit beta;Follicle-stimulating hormone beta subunit;FSH-B;FSH-beta;Follitropin beta chain;FSHB; FSHB HH24 follicle stimulating hormone subunit beta follitropin subunit beta|FSH-B|FSH-beta|follicle stimulating hormone beta subunit|follicle stimulating hormone, beta polypeptide|follitropin, beta chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FSHB, check out the FSHB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FSHB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FSH beta/FSHB Antibody Picoband® (PB9106)
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Customer Q&As
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1 Customer Q&As for Anti-FSH beta/FSHB Antibody Picoband®
Question
We are currently using anti-FSH beta/FSHB antibody PB9106 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-12
Answer
The anti-FSH beta/FSHB antibody (PB9106) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-12
