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Product Info Summary
| SKU: | A08826-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-FRMD6/Willin Antibody Picoband®
SKU/Catalog Number
A08826-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FRMD6/Willin Antibody Picoband® catalog # A08826-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FRMD6/Willin Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08826-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human FRMD6/Willin recombinant protein (Position: M1-E95).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08826-2 is reactive to FRMD6 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
72.044kDa
Background of FRMD6
FERM domain-containing protein 6 is a protein that in humans is encoded by the FRMD6 gene. It is mapped to 14q22.1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08826-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human Hela whole cell, human PC-3 whole cell, human PANC-1 whole cell, human CACO-2 whole cell, rat PC-12 whole cell, mouse Neuro-2a whole cell,
IHC: human rectum cancer tissue
ICC/IF: A431 cell
FCM: A431 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FRMD6 using anti-FRMD6 antibody (A08826-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human PANC-1 whole cell lysates,
Lane 4: human CACO-2 whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse Neuro-2a whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FRMD6 antigen affinity purified polyclonal antibody (Catalog # A08826-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FRMD6 at approximately 72KD. The expected band size for FRMD6 is at 72KD.
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Figure 2. IHC analysis of FRMD6 using anti-FRMD6 antibody (A08826-2).
FRMD6 was detected in paraffin-embedded section of human rectum cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FRMD6 Antibody (A08826-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IF analysis of FRMD6 using anti-FRMD6 antibody (A08826-2).
FRMD6 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-FRMD6 Antibody (A08826-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 4. Flow Cytometry analysis of A431 cells using anti-FRMD6 antibody (A08826-2).
Overlay histogram showing A431 cells stained with A08826-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FRMD6 Antibody (A08826-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For FRMD6 (Source: Uniprot.org, NCBI)
Gene Name
FRMD6
Full Name
FERM domain-containing protein 6
Weight
72.044kDa
Alternative Names
FERM domain-containing protein 6; Willin; FRMD6; C14orf31 FRMD6 C14orf31, EX1, Willin, c14_5320 FERM domain containing 6 FERM domain-containing protein 6|4.1 ezrin radixin moesin (FERM)-containing protein|expanded homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FRMD6, check out the FRMD6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FRMD6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FRMD6/Willin Antibody Picoband® (A08826-2)
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