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Product Info Summary
| SKU: | M03927-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-FRA1 Rabbit Monoclonal Antibody
View all FRA-1/FOSL1 Antibodies
SKU/Catalog Number
M03927-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-FRA1 Rabbit Monoclonal Antibody catalog # M03927-1. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FRA1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03927-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
24F71
Isotype
IgG
Immunogen
A synthesized peptide derived from human FRA1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03927-1 is reactive to FOSL1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
39 kDa
Calculated molecular weight
55990 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03927-1 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
Positive Control
WB: human MOLT-4 whole cell, human A431 whole cell, human U251 whole cell, human A549 whole cell, human SIHA whole cell, human U20S whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FRA1 using anti-FRA1 antibody (M03927-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MOLT-4 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human SIHA whole cell lysates,
Lane 6: human U20S whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FRA1 antigen affinity purified monoclonal antibody (Catalog # M03927-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FRA1 at approximately 39 kDa. The expected band size for FRA1 is at 29 kDa.
Protein Target Info & Infographic
Gene/Protein Information For FOSL1 (Source: Uniprot.org, NCBI)
Gene Name
FOSL1
Full Name
Fos-related antigen 1
Weight
55990 MW
Superfamily
bZIP family
Alternative Names
Perilipin-1;Lipid droplet-associated protein;PLIN1;PERI, PLIN; FOSL1 FRA, FRA1, fra-1 FOS like 1, AP-1 transcription factor subunit fos-related 1|FOS like 1, AP-1 trancription factor subunit|FOS-like -1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOSL1, check out the FOSL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOSL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FRA1 Rabbit Monoclonal Antibody (M03927-1)
Hello CJ!
M03927-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Fra-1 is upregulated in lung cancer tissues and inhibits the apoptosis of lung cancer cells by the P53 signaling pathway
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