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Product Info Summary
| SKU: | A02509 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-FPR1/Fpr Antibody
SKU/Catalog Number
A02509
Size
100ul
Form
Liquid
Description
Boster Bio Anti-FPR1/Fpr Antibody (Catalog# A02509). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FPR1/Fpr Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02509)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human FPR1. Immunogen sequence location: 155 - 204
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A02509 is reactive to FPR1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
38446 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02509 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:5000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from K562 cells, using FPR1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of MCF7 cells, using FPR1 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For FPR1 (Source: Uniprot.org, NCBI)
Gene Name
FPR1
Full Name
fMet-Leu-Phe receptor
Weight
38446 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
FPR1;fMet-Leu-Phe receptor;fMLP receptor;N-formyl peptide receptor;FPR;N-formylpeptide chemoattractant receptor FPR1 FMLP, FPR formyl peptide receptor 1 fMet-Leu-Phe receptor|N-formylpeptide chemoattractant receptor|fMLP receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FPR1, check out the FPR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FPR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FPR1/Fpr Antibody (A02509)
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No publications found for A02509
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Customer Q&As
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6 Customer Q&As for Anti-FPR1/Fpr Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-FPR1/Fpr antibody A02509. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-03
Question
Here is the WB image, lot number and protocol we used for kidney using anti-FPR1/Fpr antibody A02509. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-05
Question
Does A02509 anti-FPR1/Fpr antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Moore
Verified customer
Asked: 2019-08-02
Answer
You can see on the product datasheet, A02509 anti-FPR1/Fpr antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-02
Question
We are currently using anti-FPR1/Fpr antibody A02509 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
The anti-FPR1/Fpr antibody (A02509) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-18
Question
I was wanting to use your anti-FPR1/Fpr antibody for IF for human kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human kidney identification?
T. Yang
Verified customer
Asked: 2016-03-29
Answer
It shows on the product datasheet, A02509 anti-FPR1/Fpr antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-03-29
Question
I see that the anti-FPR1/Fpr antibody A02509 works with IF, what is the protocol used to produce the result images on the product page?
W. Krishna
Verified customer
Asked: 2015-09-21
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-09-21
