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Product Info Summary
| SKU: | A05389-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-FOX2/RBM9/RBFOX2 Antibody Picoband®
SKU/Catalog Number
A05389-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-FOX2/RBM9/RBFOX2 Antibody Picoband® catalog # A05389-2. Tested in ELISA, IF, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-FOX2/RBM9/RBFOX2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05389-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human FOX2/RBM9/RBFOX2 recombinant protein (Position: M1-Y256).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05389-2 is reactive to RBFOX2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
39411 MW
Background of FOX2
RNA binding motif protein 9 (RBM9), also known as Rbfox2, is a protein which in humans is encoded by the RBM9 gene. It is mapped to 22q12.3. This gene is one of several human genes similar to the C. elegans gene Fox-1. This gene encodes an RNA binding protein that is thought to be a key regulator of alternative exon splicing in the nervous system and other cell types. The protein binds to a conserved UGCAUG element found downstream of many alternatively spliced exons and promotes inclusion of the alternative exon in mature transcripts. The protein also interacts with the estrogen receptor 1 transcription factor and regulates estrogen receptor 1 transcriptional activity. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05389-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human U2OS whole cell, human Caco-2 whole cell, human A431 whole cell, human U-87MG whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell
IHC: human liver cancer tissue, human esophageal squamous carcinoma tissue, human lung cancer tissue, human cortical adenoma tissue, human ovarian serous adenocarcinoma tissue, mouse kidney tissue
IF: human lung cancer tissue
FCM: SH-SY5Y cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U2OS whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human U-87MG whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOX2/RBM9/RBFOX2 antigen affinity purified polyclonal antibody (Catalog # A05389-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOX2/RBM9/RBFOX2 at approximately 60 kDa. The expected band size for FOX2/RBM9/RBFOX2 is at 41 kDa.
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Figure 2. IHC analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2).
FOX2/RBM9/RBFOX2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOX2/RBM9/RBFOX2 Antibody (A05389-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2).
FOX2/RBM9/RBFOX2 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOX2/RBM9/RBFOX2 Antibody (A05389-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2).
FOX2/RBM9/RBFOX2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOX2/RBM9/RBFOX2 Antibody (A05389-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 8. IF analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2).
FOX2/RBM9/RBFOX2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-FOX2/RBM9/RBFOX2 Antibody (A05389-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 5. IHC analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2).
FOX2/RBM9/RBFOX2 was detected in a paraffin-embedded section of human cortical adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOX2/RBM9/RBFOX2 Antibody (A05389-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 9. Flow Cytometry analysis of SH-SY5Y cells using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2).
Overlay histogram showing SH-SY5Y cells stained with A05389-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOX2/RBM9/RBFOX2 Antibody (A05389-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Figure 6. IHC analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2).
FOX2/RBM9/RBFOX2 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOX2/RBM9/RBFOX2 Antibody (A05389-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2).
FOX2/RBM9/RBFOX2 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOX2/RBM9/RBFOX2 Antibody (A05389-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For RBFOX2 (Source: Uniprot.org, NCBI)
Gene Name
RBFOX2
Full Name
RNA binding protein fox-1 homolog 2
Weight
39411 MW
Alternative Names
RNA binding protein fox-1 homolog 2; Fox-1 homolog B; Hexaribonucleotide-binding protein 2; RNA-binding motif protein 9; RNA-binding protein 9; Repressor of tamoxifen transcriptional activity; RBFOX2; FOX2; HRNBP2; RBM9; RTA RBFOX2 FOX2, Fox-2, HNRBP2, HRNBP2, RBM9, RTA, dJ106I20.3, fxh RNA binding fox-1 homolog 2 RNA binding protein fox-1 homolog 2|RNA binding protein, fox-1 homolog 2|RNA-binding motif protein 9|fox-1 homolog B|fox-1 homologue|hexaribonucleotide-binding protein 2|repressor of tamoxifen transcriptional activity
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RBFOX2, check out the RBFOX2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RBFOX2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FOX2/RBM9/RBFOX2 Antibody Picoband® (A05389-2)
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