Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | M02165-1 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Dog, Hamster, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-FKBP52 Monoclonal Antibody
View all FKBP52/FKBP4 Antibodies
SKU/Catalog Number
M02165-1
Size
100ug
Form
liquid
Description
Boster Bio Anti-FKBP52 Monoclonal Antibody catalog # M02165-1. Tested in IP, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FKBP52 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02165-1)
Host
Mouse
Contents
PBS, 50% glycerol, 0.09% sodium azide
Clonality
Monoclonal
Clone Number
Hi52C
Isotype
IgG2a
Immunogen
Synthetic peptide corresponding to the residues of human FKBP52
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~52kDa. Heavy chain migrates close to FKBP52 on SDS PAGE.
Reactive Species
M02165-1 is reactive to FKBP52 in Dog, Hamster, Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
45080 MW
Background of FKBP52/FKBP4
HSP90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of HSP90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an HSP90 binding site. Among the TPR cochaperones of HSP90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the HSP90 (4). Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different HSP90-binding characteristics (3). FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02165-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB (1:2000), IHC (1:250), ICC/IF (1:1000), IP (5µg); optimal dilutions for assays should be determined by the user.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC analysis of FKBP4 using anti-FKBP4 antibody (M02165-1).
FKBP4 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-FKBP4 Antibody (M02165-1) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C (M02165-1) . Tissue: MCF-7 cells (metastatic mammary gland/breast cell line) . Species: Human. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody (M02165-1) at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red) . Courtesy of: Tom Ratajczak, Univ. of W. Australia.
Click image to see more details
Figure 2. IHC analysis of FKBP4 using anti-FKBP4 antibody (M02165-1).
FKBP4 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-FKBP4 Antibody (M02165-1) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 3. Western blot analysis of FKBP4 using anti-FKBP4 antibody (M02165-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FKBP4 antigen affinity purified polyclonal antibody (Catalog # M02165-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for FKBP4.
Click image to see more details
Figure 5. Western blot analysis of FKBP4 using anti-FKBP4 antibody (M02165-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FKBP4 antigen affinity purified polyclonal antibody (Catalog # M02165-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for FKBP4.
Protein Target Info & Infographic
Gene/Protein Information For FKBP52 (Source: Uniprot.org, NCBI)
Gene Name
FKBP52
Full Name
Peptidyl-prolyl cis-trans isomerase FKBP4
Weight
45080 MW
Alternative Names
FK506 binding protein 4, FKBP4, FKBP59, HBI, HSP56, p52, p59, PPIase, Rotamase, T cell FK506 binding protein FKBP4 FKBP51, FKBP52, FKBP59, HBI, Hsp56, PPIase, p52 FKBP prolyl isomerase 4 peptidyl-prolyl cis-trans isomerase FKBP4|FK506 binding protein 4, 59kDa|HSP binding immunophilin|T-cell FK506-binding protein, 59kD|peptidylprolyl cis-trans isomerase|rotamase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FKBP52, check out the FKBP52 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FKBP52: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FKBP52 Monoclonal Antibody (M02165-1)
Hello CJ!
No publications found for M02165-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-FKBP52 Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-FKBP52 Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-FKBP52 Monoclonal Antibody
Question
Will anti-FKBP52 Monoclonal antibody M02165-1 work on primate IF with mammary carcinoma ovarian carcinoma?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
Our lab technicians have not tested anti-FKBP52 Monoclonal antibody M02165-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-FKBP52 Monoclonal antibody M02165-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate mammary carcinoma ovarian carcinoma in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-02
Question
Would M02165-1 anti-FKBP52 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
You can see on the product datasheet, M02165-1 anti-FKBP52 Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-14
Question
We are currently using anti-FKBP52 Monoclonal antibody M02165-1 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
G. Li
Verified customer
Asked: 2018-09-14
Answer
The anti-FKBP52 Monoclonal antibody (M02165-1) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-14
