Key=store_id Key=entity_id, value=89032 Key=attribute_set_id, value=4 Key=type_id, value=simple Key=sku, value=M02317 Key=has_options, value=1 Key=required_options, value=1 Key=created_at, value=2020-03-11 06:19:30 Key=updated_at, value=2024-10-19 05:12:17 Key=mst_search_weight, value=0 Key=status, value=Enabled Key=name, value=Anti-Fibulin-5 FBLN5 Monoclonal Antibody Key=cj_search_index Key=publication_count, value=0 Key=qty_in_warehouses Key=is_recurring, value=No Key=visibility, value=Catalog, Search Key=tax_class_id, value=Taxable Goods Key=featured, value=No Key=featured_image, value=Yes Key=fb_product, value= Key=custom_product_page, value=No Key=free_shipping, value=No Key=template, value=antibodies Key=host, value=Mouse Key=quantity_and_stock_status Key=googleshopping_exclude, value=No Key=yoast_keyword_score, value=-1356 Key=yoast_content_score, value=90 Key=price, value=380.0000 Key=weight, value=1.0000 Key=meta_title, value=Anti-Fibulin-5 FBLN5 Monoclonal Antibody Key=meta_description, value=Boster Bio Anti-Fibulin-5 FBLN5 Monoclonal Antibody catalog # M02317. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. Supplied as 100ul in Liquid form antibody. Key=image, value=/antibody/M02134-lgr4-antibody-1-wb-4x3.jpg Key=small_image, value=/antibody/M02134-lgr4-antibody-1-wb-4x3.jpg Key=thumbnail, value=/antibody/M02134-lgr4-antibody-1-wb-4x3.jpg Key=url_key, value=anti-fibulin-5-fbln5-monoclonal-antibody-m02317-boster Key=url_path, value=anti-lgr4-antibody-m02134-boster Key=options_container, value=Block after Info Column Key=image_label, value=LGR4 was detected in paraffin-embedded sections of human cerebellum tissues (A) and human Pancreas islet tissues (B) using mouse anti-LGR4 Antigen Affinity purified monoclonal antibody (Catalog # M02134) at 5 μg/mL. Personal communication, Andrew Elst Key=small_image_label, value=LGR4 was detected in paraffin-embedded sections of human cerebellum tissues (A) and human Pancreas islet tissues (B) using mouse anti-LGR4 Antigen Affinity purified monoclonal antibody (Catalog # M02134) at 5 μg/mL. Personal communication, Andrew Elst Key=thumbnail_label, value=LGR4 was detected in paraffin-embedded sections of human cerebellum tissues (A) and human Pancreas islet tissues (B) using mouse anti-LGR4 Antigen Affinity purified monoclonal antibody (Catalog # M02134) at 5 μg/mL. Personal communication, Andrew Elst Key=msrp_display_actual_price_type, value=Use config Key=gift_message_available, value=No Key=size, value=100ul Key=protein_name, value=Fibulin-5 Key=gene_full_name, value=Fibulin-5 Key=uniprot_id, value=Q9UBX5 Key=sensitivity Key=applications, value=ELISA, IHC, WB Key=reactivity, value=Human Key=form, value=Liquid Key=concentration, value=1 mg/ml Key=cross_reactivity, value=No cross reactivity with other proteins. Key=clone_number, value=3F10A5 / 3F8A12 Key=predicted_reactivity Key=source_company, value=Huafeng (order through China) HF41197, cost 100ul 1200RMB Key=isotype, value=IgG Key=molecular_weight, value=50180 MW Key=sample_size_available, value=25ug for $99, contact us for details Key=related_product_skus, value=PA1611,PB9603,PROTP14902,PROTP14902-1 Key=product_category, value=Primary Antibodies, Monoclonal Antibodies Key=research_category, value=G Protein Signaling, Signal Transduction, Signaling Pathway Key=clonality, value=Monoclonal Key=swatch_image, value=/m/0/m01615-anti-slug-snai2-monoclonal-antibody-wb-testing-1_1.jpg Key=rating_value, value=100 Key=review_count, value=4 Key=mp_exclude_sitemap, value=No Key=yoast_facebook_image, value=/m/0/m01615-anti-slug-snai2-monoclonal-antibody-wb-testing-1_1.jpg Key=yoast_twitter_image, value=/m/0/m01615-anti-slug-snai2-monoclonal-antibody-wb-testing-1_1.jpg Key=cj_related_products, value=Elastic Fiber Assembly:PB9719|PA2159|A07304^Pelvic Organ Prolapse:PB9218^Pulmonary Emphysema:RP1089^Uterine Prolapse:PB9719 Key=description, value=Boster Bio Anti-Fibulin-5 FBLN5 Monoclonal Antibody catalog # M02317. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. Key=short_description, value=Boster Bio Anti-Fibulin-5 FBLN5 Monoclonal Antibody catalog # M02317. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. Key=meta_keyword, value=Anti-Fibulin-5 FBLN5 Monoclonal Antibody Key=contents, value=Ascitic fluid containing 0.03% sodium azide. Key=background Key=gene_name, value=FBLN5 Key=synonyms, value=Fibulin-5;FIBL-5;Developmental arteries and neural crest EGF-like protein;Dance;Urine p50 protein;UP50;FBLN5;DANCE;UNQ184/PRO210;
Key=application_details, value=WB 1:500-1:2000 IHC 1:200-1:1000 ELISA 1:10000 Key=subcellular_localization, value=Secreted Key=tissue_specificity, value=Expressed in skin fibroblasts (at protein
level) (PubMed:17035250). Expressed predominantly in heart, ovary,
and colon but also in kidney, pancreas, testis, lung and placenta.
Not detectable in brain, liver, thymus, prostate, or peripheral
blood leukocytes (PubMed:10428823). Key=protein_function, value=Essential for elastic fiber formation, is involved in
the assembly of continuous elastin (ELN) polymer and promotes the
interaction of microfibrils and ELN (PubMed:18185537). Stabilizes
and organizes elastic fibers in the skin, lung and vasculature (By
similarity). Promotes adhesion of endothelial cells through
interaction of integrins and the RGD motif. Vascular ligand for
integrin receptors which may play a role in vascular development
and remodeling (PubMed:10428823). May act as an adapter that
mediates the interaction between FBN1 and ELN (PubMed:17255108).
Key=application_notes, value=Anti-LGR4 monoclonal antibody has been tested by ELISA, SDS-Page, and western blot, and is suitable in immunohistochemistry. Expect a band approximately 102 kDa in size corresponding to LGR4 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required. Key=competitor_equivalent_skus, value=sc 68579|sc 68578|sc 292344|sc 390630 Key=immunogen, value=Purified recombinant fragment of Fibulin-5 expressed in E. Coli. Key=purification, value=Affinity purification Key=storage, value=Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Key=options Key=media_gallery Key=extension_attributes Key=tier_price Key=tier_price_changed Key=category_ids Key=is_salable Key=website_ids Key=request_path Key=rating_summary Key=_cache_instance_store_filter Key=media_gallery_images
Anti-Fibulin-5 FBLN5 Monoclonal Antibody
Fibulin 5/DANCE antibody
Boster Bio Anti-Fibulin-5 FBLN5 Monoclonal Antibody catalog # M02317. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Boster Bio Anti-Fibulin-5 FBLN5 Monoclonal Antibody catalog # M02317. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fibulin-5 FBLN5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02317)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
3F10A5 / 3F8A12
Isotype
IgG
Immunogen
Purified recombinant fragment of Fibulin-5 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M02317 is reactive to FBLN5 in Human
Reconstitution
Calculated molecular weight
50180 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000 IHC 1:200-1:1000 ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of FBLN5 using Anti-Fibulin-5 FBLN5 Monoclonal Antibody (M02317).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Stomach carcinoma (left) and breast carcinoma (right)
Click image to see more details
Figure 1. Western blotting validation for Anti-Fibulin-5 FBLN5 Monoclonal Antibody M02317
Western Blot (WB) analysis using Fibulin-5 Monoclonal Antibody against truncated fibulin5 recombinant protein (1) and HeLa cell lysate (2).
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Gene/Protein Information For FBLN5 (Source: Uniprot.org, NCBI)
Gene Name
FBLN5
Full Name
Fibulin-5
Weight
50180 MW
Superfamily
fibulin family
Alternative Names
Fibulin-5;FIBL-5;Developmental arteries and neural crest EGF-like protein;Dance;Urine p50 protein;UP50;FBLN5;DANCE;UNQ184/PRO210;
FBLN5 ADCL2, ARCL1A, ARMD3, DANCE, EVEC, FIBL-5, HNARMD, UP50 fibulin 5 fibulin-5|developmental arteries and neural crest EGF-like protein|embryonic vascular EGF-like repeat-containing protein|testis tissue sperm-binding protein Li 75n|urine p50 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on FBLN5, check out the FBLN5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FBLN5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$380.00
Troubleshooting
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.