Anti-Fibroblast Activation Protein FAP Antibody

Product Name

Anti-Fibroblast Activation Protein FAP Antibody

View all Fibroblast Activation Protein alpha/FAP Antibodies

SKU/Catalog Number

A00422

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Fibroblast Activation Protein FAP Antibody (Catalog # A00422). Tested in ELISA, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Fibroblast Activation Protein FAP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00422)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse FAP protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00422 is reactive to FAP in Mouse

Reconstitution

Calculated molecular weight

87713 MW

Background of Fibroblast Activation Protein alpha/FAP

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Fibroblast Activation Protein (FAP)  is expressed in the stroma of sites that are undergoing wound healing. In addition, it has recently been reported that FAP is expressed in the stroma of sites of metastatic disease. Inhibition of FAP may lead to a dramatic decrease in the number of metastatic osteosarcoma lung nodules.  FAP exists as an inactive monomer and when activated forms homodimers or heterodimers with DPP4. Multiple isoforms of FAP are reported as alternative splicing products from a common gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A00422 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:20,000
WB: 1:500 to 1:2,000

Validation Images & Assay Conditions

Gene/Protein Information For FAP (Source: Uniprot.org, NCBI)

Alternative Names

Fap, Fibroblast activation protein alpha, Prolyl endopeptidase FAP, Dipeptidyl peptidase FAP, Integral membrane serine protease, FAPA, Gelatine degradation protease FAP, Surface-expressed protease, SIMP, Seprase, APCE FAP DPPIVA, FAPalpha, SIMP, FAP fibroblast activation protein alpha prolyl endopeptidase FAP|170 kDa melanoma membrane-bound gelatinase|dipeptidyl peptidase FAP|gelatine degradation protease FAP|integral membrane serine protease|post-proline cleaving enzyme|seprase|surface-expressed protease

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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