Click image to see more details
Product Info Summary
| SKU: | M00422 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-FAP1 Rabbit Monoclonal Antibody
View all Fibroblast Activation Protein alpha/FAP Antibodies
SKU/Catalog Number
M00422
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-FAP1 Rabbit Monoclonal Antibody catalog # M00422. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FAP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00422)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AbF22
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FAP1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00422 is reactive to FAP in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
95 kDa
Calculated molecular weight
87713 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00422 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of FAP1 using anti-FAP1 antibody (M00422).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAP1 antigen affinity purified monoclonal antibody (Catalog # M00422) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAP1 at approximately 95 kDa. The expected band size for FAP1 is at 86 kDa.
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For FAP (Source: Uniprot.org, NCBI)
Gene Name
FAP
Full Name
Prolyl endopeptidase FAP
Weight
87713 MW
Superfamily
peptidase S9B family
Alternative Names
Prolyl endopeptidase FAP ;3.4.21.26 ;170 kDa melanoma membrane-bound gelatinase ;Dipeptidyl peptidase FAP ;3.4.14.5 ;Fibroblast activation protein alpha ;FAPalpha ;Gelatine degradation protease FAP ;3.4.21.- ;Integral membrane serine protease ;Post-proline cleaving enzyme ;Serine integral membrane protease ;SIMP ;Surface-expressed protease ;Seprase ;Antiplasmin-cleaving enzyme FAP, soluble form ;APCE ;3.4.14.5 ;3.4.21.- ;3.4.21.26 ;FAP ; FAP DPPIVA, FAPalpha, SIMP, FAP fibroblast activation protein alpha prolyl endopeptidase FAP|170 kDa melanoma membrane-bound gelatinase|dipeptidyl peptidase FAP|gelatine degradation protease FAP|integral membrane serine protease|post-proline cleaving enzyme|seprase|surface-expressed protease
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FAP, check out the FAP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FAP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FAP1 Rabbit Monoclonal Antibody (M00422)
Hello CJ!
No publications found for M00422
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-FAP1 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-FAP1 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-FAP1 Rabbit Monoclonal Antibody
Question
We are currently using anti-FAP1 Rabbit Monoclonal antibody M00422 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
The anti-FAP1 Rabbit Monoclonal antibody (M00422) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-19
Question
My team were content with the WB result of your anti-FAP1 Rabbit Monoclonal antibody. However we have seen positive staining in fibroblast isoform 2: cytoplasm using this antibody. Is that expected? Could you tell me where is FAP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
According to literature, fibroblast does express FAP. Generally FAP expresses in prolyl endopeptidase fap: cell surface, antiplasmin-cleaving enzyme fap, soluble, isoform 2: cytoplasm. Regarding which tissues have FAP expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 7911242
Melanoma, Pubmed ID: 9065413, 9247085, 10644713
Placenta, Pubmed ID: 15489334
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2019-10-11
Question
Can M00422 antibody be used for IHC-P application?
Verified customer
Asked: 2019-07-05
Answer
The anti-FAP1 Rabbit Monoclonal Antibody (M00422) can be used for IHC-P application on human.
Boster Scientific Support
Answered: 2019-07-08
Question
We ordered your anti-FAP1 Rabbit Monoclonal antibody for WB on melanoma in a previous project. I am using human, and We intend to use the antibody for IHC next. I am looking for examining melanoma as well as placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2018-05-17
Answer
I have checked the website and datasheets of our anti-FAP1 Rabbit Monoclonal antibody and it seems that M00422 has been validated on human in both WB and IHC. Thus M00422 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-05-17
Question
We have observed staining in human placenta. Any tips? Is anti-FAP1 Rabbit Monoclonal antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2017-05-25
Answer
From what I have seen in literature placenta does express FAP. From what I have seen in Uniprot.org, FAP is expressed in body of uterus, fibroblast, melanoma, placenta, plasma, among other tissues. Regarding which tissues have FAP expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 7911242
Melanoma, Pubmed ID: 9065413, 9247085, 10644713
Placenta, Pubmed ID: 15489334
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2017-05-25
