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Product Info Summary
| SKU: | M00816-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Fibrinogen alpha chain Rabbit Monoclonal Antibody
SKU/Catalog Number
M00816-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Fibrinogen alpha chain Rabbit Monoclonal Antibody catalog # M00816-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fibrinogen alpha chain Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00816-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
23F47
Isotype
IgG
Immunogen
A synthesized peptide derived from human Fibrinogen alpha chain
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00816-1 is reactive to FGA in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
95 kDa
Calculated molecular weight
95 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00816-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: human hepatocellular carcinoma tumor tissue (HCCT), human hepatocellular carcinoma paracancerous tissue (HCCP), human HepG2 whole cell, rat liver tissue, rat RH35 whole cell, mouse liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Fibrinogen Alpha Chain using anti-Fibrinogen Alpha Chain antibody (M00816-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat RH35 whole cell lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fibrinogen Alpha Chain antigen affinity purified monoclonal antibody (Catalog # M00816-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fibrinogen Alpha Chain at approximately 95 kDa. The expected band size for Fibrinogen Alpha Chain is at 95 kDa.
Protein Target Info & Infographic
Gene/Protein Information For FGA (Source: Uniprot.org, NCBI)
Gene Name
FGA
Full Name
Fibrinogen alpha chain
Weight
95 kDa
Alternative Names
Scavenger receptor cysteine-rich type 1 protein M130;Hemoglobin scavenger receptor;CD163;Soluble CD163;sCD163;CD163;M130; FGA Fib2 fibrinogen alpha chain fibrinogen alpha chain|fibrinogen, A alpha polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FGA, check out the FGA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FGA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fibrinogen alpha chain Rabbit Monoclonal Antibody (M00816-1)
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