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Product Info Summary
| SKU: | A01258-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-FHL1 Antibody Picoband®
SKU/Catalog Number
A01258-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FHL1 Antibody Picoband® catalog # A01258-3. Tested in ELISA, WB applications. This antibody reacts with Human， Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-FHL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01258-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human FHL1 recombinant protein (Position: K133-Q179).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01258-3 is reactive to FHL1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
36.263kDa
Background of FHL1
Four and a half LIM domains protein 1 is a protein that in humans is encoded by the FHL1 gene. This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01258-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat heart tissue, mouse heart tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FHL1 using anti-FHL1 antibody (A01258-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHL1 antigen affinity purified polyclonal antibody (Catalog # A01258-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHL1 at approximately 36 kDa. The expected band size for FHL1 is at 36 kDa.
Protein Target Info & Infographic
Gene/Protein Information For FHL1 (Source: Uniprot.org, NCBI)
Gene Name
FHL1
Full Name
Four and a half LIM domains protein 1
Weight
36.263kDa
Alternative Names
Four and a half LIM domains protein 1; FHL-1; KyoT; RBP-associated molecule 14-1; RAM14-1; Skeletal muscle LIM-protein 1; SLIM; SLIM-1 FHL1 FCMSU, FHL-1A, FHL1B, FLH1A, KYOT, RBMX1A, RBMX1B, SLIM, SLIM-1, SLIM1, SLIMMER, XMPMA, FHL1 four and a half LIM domains 1 four and a half LIM domains protein 1|LIM protein SLIMMER|four-and-a-half Lin11, Isl-1 and Mec-3 domains 1|skeletal muscle LIM-protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FHL1, check out the FHL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FHL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FHL1 Antibody Picoband® (A01258-3)
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