Product Info Summary
SKU: | M01258 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, IHC, WB |
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Product info
Product Name
Anti-FHL1 Monoclonal Antibody
SKU/Catalog Number
M01258
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-FHL1 Monoclonal Antibody catalog # M01258. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FHL1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01258)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFCI-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FHL1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01258 is reactive to FHL1 in Human, Mouse, Rat
Applications
M01258 is guaranteed for IP, IHC, WB Boster Guarantee
Observed Molecular Weight
85 kDa
Calculated molecular weight
50688 MW
Background of FHL1
Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
![m01258 wb m01258 wb](https://www.bosterbio.com/media/catalog/product/m/0/m01258-wb.jpg)
Click image to see more details
Western blot analysis of FHL1 expression in Human fetal muscle lysate.
Protein Target Info & Infographic
Gene/Protein Information For FHL1 (Source: Uniprot.org, NCBI)
Gene Name
FHL1
Full Name
Four and a half LIM domains protein 1
Weight
50688 MW
Alternative Names
bA535K18.1; FHL1; four and a half LIM domains 1; HLH1; HPLH1; KYOT; KYO-T; LIM protein SLIMMER; skeletal muscle LIM-protein1; SLIM1; SLIM1Isl-1 and Mec-3 domains 1; SLIMMER; XMPMA FHL1 FCMSU, FHL-1A, FHL1B, FLH1A, KYOT, RBMX1A, RBMX1B, SLIM, SLIM-1, SLIM1, SLIMMER, XMPMA, FHL1 four and a half LIM domains 1 four and a half LIM domains protein 1|LIM protein SLIMMER|four-and-a-half Lin11, Isl-1 and Mec-3 domains 1|skeletal muscle LIM-protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FHL1, check out the FHL1 Infographic
![FHL1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FHL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FHL1 Monoclonal Antibody (M01258)
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No publications found for M01258
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-FHL1 Monoclonal Antibody
Question
What is the sample target on Western blot analysis in your product image for M01258?
Verified customer
Asked: 2019-12-09
Answer
Western blot analysis of the Anti-FHL1 Monoclonal Antibody (M01258) expression in Human fetal muscle lysate.
Boster Scientific Support
Answered: 2019-12-10
Question
I see that the anti-FHL1 Monoclonal antibody M01258 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-05
Question
Will anti-FHL1 Monoclonal antibody M01258 work on zebrafish WB with brain?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
Our lab technicians have not validated anti-FHL1 Monoclonal antibody M01258 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-FHL1 Monoclonal antibody M01258 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-20
Question
I have a question about product M01258, anti-FHL1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01258 anti-FHL1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-24
Question
Is this M01258 anti-FHL1 Monoclonal antibody reactive to the isotypes of FHL1?
Verified Customer
Verified customer
Asked: 2017-10-26
Answer
The immunogen of M01258 anti-FHL1 Monoclonal antibody is A synthesized peptide derived from human FHL1 May have an involvement in muscle development or hypertrophy. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-26
Question
Does M01258 anti-FHL1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-23
Answer
As indicated on the product datasheet, M01258 anti-FHL1 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-23
Question
I am looking for to test anti-FHL1 Monoclonal antibody M01258 on rat bone marrow for research purposes, then I may be interested in using anti-FHL1 Monoclonal antibody M01258 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
T. Singh
Verified customer
Asked: 2016-04-15
Answer
The products we sell, including anti-FHL1 Monoclonal antibody M01258, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-04-15