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Product Info Summary
| SKU: | A00790-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, IHC-F, ICC, WB |
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Product info
Product Name
Anti-Fibrinogen gamma chain/FGG Antibody Picoband®
View all Fibrinogen gamma chain Antibodies
SKU/Catalog Number
A00790-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Fibrinogen gamma chain/FGG Antibody Picoband® catalog # A00790-1. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fibrinogen gamma chain/FGG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00790-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human FGG, different from the related mouse sequence by two amino acids, and from the related rat sequence by five amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A00790-1 is reactive to FGG in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
52 kDa
Calculated molecular weight
51512 MW
Background of Fibrinogen gamma chain
Fibrinogen gamma chain, also known as FGG, is a human gene found on Chromosome 4. The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00790-1 is guaranteed for Flow Cytometry, IHC, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human HepG2 whole cell, human hepatocellular carcinoma tumor tissue (HCCT), human hepatocellular carcinoma paracancerous tissue (HCCP), rat liver tissue, mouse liver tissue
IHC: human liver cancer tissue
FCM: SiHa cell, A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FGG using anti-FGG antibody (A00790-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
Lane 3: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,
Lane 4: rat liver tissue lysates,
Lane 5: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGG antigen affinity purified polyclonal antibody (Catalog # A00790-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGG at approximately 52 kDa. The expected band size for FGG is at 52 kDa.
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Figure 2. IHC analysis of FGG using anti-FGG antibody (A00790-1).
FGG was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FGG Antibody (A00790-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. Flow Cytometry analysis of SiHa cells using anti-FGG antibody (A00790-1).
Overlay histogram showing SiHa cells stained with A00790-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FGG Antibody (A00790-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 4. Flow Cytometry analysis of A549 cells using anti-FGG antibody (A00790-1).
Overlay histogram showing A549 cells stained with A00790-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FGG Antibody (A00790-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For FGG (Source: Uniprot.org, NCBI)
Gene Name
FGG
Full Name
Fibrinogen gamma chain
Weight
51512 MW
Alternative Names
Fibrinogen gamma chain;FGG;PRO2061; FGG fibrinogen gamma chain fibrinogen gamma chain|fibrinogen, gamma polypeptide|testicular tissue protein Li 70
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FGG, check out the FGG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FGG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fibrinogen gamma chain/FGG Antibody Picoband® (A00790-1)
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Customer Q&As
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7 Customer Q&As for Anti-Fibrinogen gamma chain/FGG Antibody Picoband®
Question
Is there a BSA free version of anti-Fibrinogen gamma chain/FGG antibody A00790-1 available?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Fibrinogen gamma chain/FGG antibody A00790-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-17
Question
We are currently using anti-Fibrinogen gamma chain/FGG antibody A00790-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
O. Walker
Verified customer
Asked: 2019-12-03
Answer
The anti-Fibrinogen gamma chain/FGG antibody (A00790-1) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-03
Question
I see that the anti-Fibrinogen gamma chain/FGG antibody A00790-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-10
Question
My lab would like to test anti-Fibrinogen gamma chain/FGG antibody A00790-1 on human platelet for research purposes, then I may be interested in using anti-Fibrinogen gamma chain/FGG antibody A00790-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-01-31
Answer
The products we sell, including anti-Fibrinogen gamma chain/FGG antibody A00790-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-31
Question
Is a blocking peptide available for product anti-Fibrinogen gamma chain/FGG antibody (A00790-1)?
T. Edwards
Verified customer
Asked: 2018-12-05
Answer
We do provide the blocking peptide for product anti-Fibrinogen gamma chain/FGG antibody (A00790-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-12-05
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for platelet using anti-Fibrinogen gamma chain/FGG antibody A00790-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-10-11
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-11
Question
Does anti-Fibrinogen gamma chain/FGG antibody A00790-1 work on canine IHC-P with skeletal muscle?
Verified Customer
Verified customer
Asked: 2018-02-26
Answer
Our lab technicians have not validated anti-Fibrinogen gamma chain/FGG antibody A00790-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Fibrinogen gamma chain/FGG antibody A00790-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine skeletal muscle in IHC-P, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-26
