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Facts about Fibrinogen gamma chain.
Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have demonstrated it is not absolutely required for thrombus formation in vivo.
Human | |
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Gene Name: | FGG |
Uniprot: | P02679 |
Entrez: | 2266 |
Belongs to: |
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No superfamily |
fibrinogen gamma chain; fibrinogen, gamma polypeptide
Mass (kDA):
51.512 kDA
Human | |
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Location: | 4q32.1 |
Sequence: | 4; NC_000004.12 (154604134..154612808, complement) |
Detected in blood plasma (at protein level).
Secreted.
What is the best application for the FGG marker in your research? It is a protein that is expressed in E. bacteria with a His-Tag and a sequence domain of 27-437aa. FGG is stable between +2degC and 8degC for a week and -20degC- -80degC during long-term storage. However repeated freeze-thaw cycles must be avoided. Biological assays are accessible to all scientists in the world.
It's easy to see the process by which this biotech company was established by studying the Historial background of Boster bio. Boster quickly earned the reputation of the man who "converts science into a toilet" after he developed his first product in 1993. Boster developed hundreds of primary antibodies as well as various IHC products. He was able to create a catalog antibody company that became the largest in China in the late nineteen nineties. Boster also created a proprietary ELISA platform, PicoKine(tm), that delivers high-sensitivity ELISA kits.
There are a variety of sources offering biological assays to detect fibrinogen-gamma. The tests are based on antibodies that are either monoclonal, or polyclonal, against this protein. Fibrinogen Gamma Chain can be found in many samples, including human and mouse. These antibodies are essential for thrombus formation.
Cell lines and tissues from CRPC exhibit elevated levels of FGG. Patients with CRPC have higher levels of FGG than patients with PCa localized. Serum FGG staining intensity is used as a guideline for CRPC diagnosis. This test can be used by physicians to determine the stage of PCa. Clinical trial participants must submit FGG staining samples to determine whether this biomarker can be used to diagnose CRPC.
In this study, we utilized DU145 cells which express shRNA that targets FGG expression, and an untreated cell line that expresses empty vector to determine the effectiveness of knockdown of the FGG gene. To confirm the efficacy of the shRNA, we performed western blots and qRTPCR analyses. Then we implanted one-x 107 cells subcutaneously under the arm of mice who were not nude. We compared the tumor volumes of FGG-KD and NC cells to determine their relative sizes.
Signaling by IL-6 is implicated in the progression of PCa. PCa patients with CRPC are more likely to have elevated levels IL-6. These increased levels could be caused by anti-androgen therapy, or through knocking down AR gene. Schroeder and colleagues studied the effects of ADT on FGG gene expression. Their findings suggest that anti-androgen therapy hinders the AR signaling, leading to an increase in FGG.
PMID: 6688357 by Chung D.W., et al. Characterization of a complementary deoxyribonucleic acid coding for the gamma chain of human fibrinogen.
PMID: 2990550 by Rixon M.W., et al. Nucleotide sequence of the gene for the gamma chain of human fibrinogen.