Product Info Summary
SKU: | M00200 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-FGFR3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00200
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-FGFR3 Rabbit Monoclonal Antibody catalog # M00200. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FGFR3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00200)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAID-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FGFR3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00200 is reactive to FGFR3 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
103 kDa
Calculated molecular weight
87710 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00200 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of FGFR3 expression in (1) A549 cell lysate; (2) Mouse brain lysate.
Protein Target Info & Infographic
Gene/Protein Information For FGFR3 (Source: Uniprot.org, NCBI)
Gene Name
FGFR3
Full Name
Fibroblast growth factor receptor 3
Weight
87710 MW
Superfamily
protein kinase superfamily
Alternative Names
Fibroblast growth factor receptor 3;FGFR-3;2.7.10.1;CD333;FGFR3;JTK4; FGFR3 ACH, CD333, CEK2, HSFGFR3EX, JTK4 fibroblast growth factor receptor 3 fibroblast growth factor receptor 3|FGFR-3|fibroblast growth factor receptor 3 variant 4|fibroblast growth factor receptor 3-S|hydroxyaryl-protein kinase|tyrosine kinase JTK4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FGFR3, check out the FGFR3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FGFR3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FGFR3 Rabbit Monoclonal Antibody (M00200)
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No publications found for M00200
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Customer Q&As
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6 Customer Q&As for Anti-FGFR3 Rabbit Monoclonal Antibody
Question
Does M00200 anti-FGFR3 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
As indicated on the product datasheet, M00200 anti-FGFR3 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-23
Question
I have a question about product M00200, anti-FGFR3 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00200 anti-FGFR3 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-FGFR3 Rabbit Monoclonal antibody M00200. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-04-30
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-30
Question
I am interested in to test anti-FGFR3 Rabbit Monoclonal antibody M00200 on mouse erythroleukemia for research purposes, then I may be interested in using anti-FGFR3 Rabbit Monoclonal antibody M00200 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-01-11
Answer
The products we sell, including anti-FGFR3 Rabbit Monoclonal antibody M00200, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-11
Question
Is a blocking peptide available for product anti-FGFR3 Rabbit Monoclonal antibody (M00200)?
S. Carter
Verified customer
Asked: 2016-12-21
Answer
We do provide the blocking peptide for product anti-FGFR3 Rabbit Monoclonal antibody (M00200). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-12-21
Question
Will anti-FGFR3 Rabbit Monoclonal antibody M00200 work for WB with erythroleukemia?
A. Kulkarni
Verified customer
Asked: 2014-04-04
Answer
According to the expression profile of erythroleukemia, FGFR3 is highly expressed in erythroleukemia. So, it is likely that anti-FGFR3 Rabbit Monoclonal antibody M00200 will work for WB with erythroleukemia.
Boster Scientific Support
Answered: 2014-04-04