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Product Info Summary
| SKU: | A00200 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-FGFR3 Antibody
SKU/Catalog Number
A00200
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-FGFR3 Antibody (Catalog # A00200). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FGFR3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00200)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human FGFR3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00200 is reactive to FGFR3 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
87710 MW
Background of FGFR3
Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. Plays an essential role in the regulation of chondrocyte differentiation, proliferation and apoptosis, and is required for normal skeleton development. Regulates both osteogenesis and postnatal bone mineralization by osteoblasts. Promotes apoptosis in chondrocytes, but can also promote cancer cell proliferation. Required for normal development of the inner ear. Phosphorylates PLCG1, CBL and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Plays a role in the regulation of vitamin D metabolism. Mutations that lead to constitutive kinase activation or impair normal FGFR3 maturation, internalization and degradation lead to aberrant signaling. Over-expressed or constitutively activated FGFR3 promotes activation of PTPN11/SHP2, STAT1, STAT5A and STAT5B. Secreted isoform 3 retains its capacity to bind FGF1 and FGF2 and hence may interfere with FGF signaling.
Jing Chen, Blood, Jul 2005; 106: 328 - 337.
Qing Zhang, Diabetes, Jan 2007; 56: 96 - 106.
Nigel P. Pringle, Development, Jan 2003; 130: 93.
Gladys Valverde-Franco, Hum. Mol. Genet., Feb 2004; 13: 271 - 284.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00200 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from LOVO cells, using FGFR3 antibody A00200.
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Western blot analysis of extracts from 293 cells (Lane 2) and HepG2 cells (Lane 3), using FGFR3 antiobdy A00200. The lane on the left is treated with synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FGFR3 antibody A00200.
Protein Target Info & Infographic
Gene/Protein Information For FGFR3 (Source: Uniprot.org, NCBI)
Gene Name
FGFR3
Full Name
Fibroblast growth factor receptor 3
Weight
87710 MW
Superfamily
protein kinase superfamily
Alternative Names
Fibroblast growth factor receptor 3;FGFR-3;2.7.10.1;CD333;FGFR3;JTK4; FGFR3 ACH, CD333, CEK2, HSFGFR3EX, JTK4 fibroblast growth factor receptor 3 fibroblast growth factor receptor 3|FGFR-3|fibroblast growth factor receptor 3 variant 4|fibroblast growth factor receptor 3-S|hydroxyaryl-protein kinase|tyrosine kinase JTK4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FGFR3, check out the FGFR3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FGFR3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FGFR3 Antibody (A00200)
Hello CJ!
A00200 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression of fibroblast growth factor 9 and its receptors in the dentate gyrus of hippocampus in poststroke depression rats
Fluoride regulates the expression of extracellular matrix HSPG and related signaling pathways FGFR3 and Ihh/PTHrP feedback loop during endochondral ossification
Nannapaneni S,Griffith CC,Magliocca KR,Chen W,Lyu X,Chen Z,Wang D,Wang X,Shin DM,Chen ZG,Saba NF.Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma.PLoS One
Species: Human
A00200 usage in article: APP:IHC, SAMPLE:FFPE TUMOR TISSUE, DILUTION:NA
Gao Y,Gui F,Li D,Zhang R,Sun Q,Guo X.Fluoride regulates the expression of extracellular matrix HSPG and related signaling pathways FGFR3 and Ihh/PTHrP feedback loop during endochondral ossification.Environ Toxicol Pharmacol.2020 Jan;73:103275.doi:10.1016/
Species: Rat,Mouse
A00200 usage in article: APP:IHC, SAMPLE:PROXIMAL TIBIA, DILUTION:1:100
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