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Product Info Summary
| SKU: | M00231-2 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-FGFR2 Mouse Monoclonal Antibody [Clone ID: OTI5C5]
SKU/Catalog Number
M00231-2
Size
100 µl
Description
Boster Bio FGFR2 mouse monoclonal antibody, clone OTI5C5 (formerly 5C5). Catalog# M00231-2. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-FGFR2 Mouse Monoclonal Antibody [Clone ID: OTI5C5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00231-2)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI5C5
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human FGFR2 (NP_000132) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00231-2 is reactive to FGFR2 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
92.025kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00231-2 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGFR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR2.
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Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-FGFR2 monoclonal antibody at 1:200.
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No Cross-Reactivity of anti-FGFR2 (M00231-2) with FGFR1
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FGFR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FGFR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FGFR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-FGFR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FGFR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either FGFR2 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FGFR2 antibody (M00231-2)
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Flow cytometric Analysis of Hela cells
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Anti-FGFR2 mouse monoclonal antibody (M00231-2) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FGFR2.
Protein Target Info & Infographic
Gene/Protein Information For FGFR2 (Source: Uniprot.org, NCBI)
Gene Name
FGFR2
Full Name
Fibroblast growth factor receptor 2
Weight
92.025kDa
Superfamily
protein kinase superfamily
Alternative Names
BBDS; BEK; BFR-1; CD332 antigen; CD332; CEK3; CFD1; craniofacial dysostosis 1; EC 2.7.10; EC 2.7.10.1; ECT1; FGF R2; FGFR2; fibroblast growth factor receptor 2; FLJ98662; Jackson-Weiss syndrome; JWS; Keratinocyte growth factor receptorreceptor like 14; KGFR; KSAM; K-sam; soluble FGFR4 variant 4; TK14; TK25 FGFR2 BBDS, BEK, BFR-1, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25 fibroblast growth factor receptor 2 fibroblast growth factor receptor 2|BEK fibroblast growth factor receptor|bacteria-expressed kinase|keratinocyte growth factor receptor|protein tyrosine kinase, receptor like 14
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FGFR2, check out the FGFR2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FGFR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FGFR2 Mouse Monoclonal Antibody [Clone ID: OTI5C5] (M00231-2)
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