Click image to see more details
Product Info Summary
| SKU: | PB10063 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-FGF21 Antibody Picoband®
SKU/Catalog Number
PB10063
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FGF21 Antibody Picoband® catalog # PB10063. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FGF21 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10063)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human FGF21 recombinant protein (Position: H29-S209). Human FGF21 shares 80.7% amino acid (aa) sequence identity with mouse FGF21.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10063 is reactive to FGF21 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22 kDa
Calculated molecular weight
22300 MW
Background of FGF-21
FGF21 (Fibroblast growth factor 21) is a protein that in humans is encoded by the FGF21 gene, which is also a member of the fibroblast growth factor (FGF) family. The FGF21 gene is mapped on 19q13.33. Using RT-PCR, Fgf21 was expressed in several types of adipose tissue in mice, including subcutaneous and epididymal fat pads and brown adipose tissue. The level of Fgf21 expression in adipose tissue was comparable to that in liver. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents. Changes in Fgf21 expression due to suckling or nutritional manipulations were associated with changes in circulating free fatty acid and ketone body levels. In differentiated mouse brown adipocytes in culture, Fgf21 treatment increased the expression of thermogenic genes, caused higher total and uncoupled respiration, and enhanced glucose oxidation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10063 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human placenta tissue,, rat spleen tissue, rat liver tissue, rat RH35 whole cell, rat brain tissue, mouse RAW2647 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of FGF21 using anti-FGF21 antibody (PB10063).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF21 antigen affinity purified polyclonal antibody (Catalog # PB10063) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF21 at approximately 22KD. The expected band size for FGF21 is at 22KD.
Click image to see more details
Figure 2. Western blot analysis of FGF21 using anti-FGF21 antibody (PB10063).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: rat RH35 whole cell lysates,
Lane 4: rat brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF21 antigen affinity purified polyclonal antibody (Catalog # PB10063) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF21 at approximately 22KD. The expected band size for FGF21 is at 22KD.
Click image to see more details
Figure 3. Western blot analysis of FGF21 using anti-FGF21 antibody (PB10063).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse RAW264.7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF21 antigen affinity purified polyclonal antibody (Catalog # PB10063) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF21 at approximately 22KD. The expected band size for FGF21 is at 22KD.
Protein Target Info & Infographic
Gene/Protein Information For FGF21 (Source: Uniprot.org, NCBI)
Gene Name
FGF21
Full Name
Fibroblast growth factor 21
Weight
22300 MW
Superfamily
heparin-binding growth factors family
Alternative Names
Fibroblast growth factor 21;FGF-21;FGF21;UNQ3115/PRO10196; FGF21 fibroblast growth factor 21 fibroblast growth factor 21
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FGF21, check out the FGF21 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FGF21: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FGF21 Antibody Picoband® (PB10063)
Hello CJ!
PB10063 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression of Fibroblast Growth Factor 21 and β-Klotho Regulates Hepatic Fibrosis through the Nuclear Factor-κB and c-Jun N-Terminal Kinase Pathways
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-FGF21 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-FGF21 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-FGF21 Antibody Picoband®
Question
Does anti-FGF21 antibody PB10063 work for ELISA with right lobe of liver?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
According to the expression profile of right lobe of liver, FGF21 is highly expressed in right lobe of liver. So, it is likely that anti-FGF21 antibody PB10063 will work for ELISA with right lobe of liver.
Boster Scientific Support
Answered: 2020-04-09
Question
Can you help my question with product PB10063, anti-FGF21 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB10063 anti-FGF21 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-18
Question
I was wanting to use your anti-FGF21 antibody for ELISA for rat right lobe of liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat right lobe of liver identification?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
It shows on the product datasheet, PB10063 anti-FGF21 antibody has been validated for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat right lobe of liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-11
Question
My boss were content with the WB result of your anti-FGF21 antibody. However we have observed positive staining in lung secreted. using this antibody. Is that expected? Could you tell me where is FGF21 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
According to literature, lung does express FGF21. Generally FGF21 expresses in secreted. Regarding which tissues have FGF21 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-11-22
Question
Do you have a BSA free version of anti-FGF21 antibody PB10063 available?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FGF21 antibody PB10063 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-09
Question
I see that the anti-FGF21 antibody PB10063 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-12
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right lobe of liver using anti-FGF21 antibody PB10063. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-18
Question
See below the WB image, lot number and protocol we used for right lobe of liver using anti-FGF21 antibody PB10063. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-20
Question
We have observed staining in rat right lobe of liver. Any tips? Is anti-FGF21 antibody supposed to stain right lobe of liver positively?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
Based on literature right lobe of liver does express FGF21. Based on Uniprot.org, FGF21 is expressed in right lobe of liver, lung, among other tissues. Regarding which tissues have FGF21 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-06-14
Question
Is this PB10063 anti-FGF21 antibody reactive to the isotypes of FGF21?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
The immunogen of PB10063 anti-FGF21 antibody is E. coli-derived human FGF21 recombinant protein (Position: H29-S209). Human FGF21 shares 80.7% amino acid (aa) sequence identity with mouse FGF21. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-13
Question
Will PB10063 anti-FGF21 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-09-03
Answer
As indicated on the product datasheet, PB10063 anti-FGF21 antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-03
Question
Our lab want to know about using your anti-FGF21 antibody for regulation of low-density lipoprotein particle clearance studies. Has this antibody been tested with western blotting on spleen tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-05-10
Answer
I appreciate your inquiry. This PB10063 anti-FGF21 antibody is tested on human placenta tissue, rat liver tissue, spleen tissue, brain tissue. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-05-10
Question
We are currently using anti-FGF21 antibody PB10063 for rat tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-18
Answer
The anti-FGF21 antibody (PB10063) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-18
Question
We ordered your anti-FGF21 antibody for WB on lung a few months ago. I am using human, and We intend to use the antibody for ELISA next. you antibody examining lung as well as right lobe of liver in our next experiment. Could give a recommendation on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2017-08-30
Answer
I have checked the website and datasheets of our anti-FGF21 antibody and it appears that PB10063 has been validated on human in both WB and ELISA. Thus PB10063 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-08-30
Question
Our lab want to know about to test anti-FGF21 antibody PB10063 on rat right lobe of liver for research purposes, then I may be interested in using anti-FGF21 antibody PB10063 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Bhatt
Verified customer
Asked: 2017-07-24
Answer
The products we sell, including anti-FGF21 antibody PB10063, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-24
Question
Is a blocking peptide available for product anti-FGF21 antibody (PB10063)?
S. Thomas
Verified customer
Asked: 2014-06-30
Answer
We do provide the blocking peptide for product anti-FGF21 antibody (PB10063). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-06-30
