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Product Info Summary
| SKU: | A04181-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-FGF-18 Antibody
SKU/Catalog Number
A04181-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-FGF-18 Antibody catalog # A04181-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FGF-18 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04181-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the C-terminal region of human FGF18. AA range:158-207
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04181-1 is reactive to FGF18 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
23989 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04181-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC-P, 1:100-300
Validation Images & Assay Conditions
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Immunohistochemistry (IHC) analysis of paraffin-embedded Mouse Lung, antibody was diluted at 1:100.
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Western Blot (WB) analysis of SW480, SKOV3, BT474, Mouse Heart cells using FGF-18 Polyclonal antibody.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Rat Lung, antibody was diluted at 1:100.
Protein Target Info & Infographic
Gene/Protein Information For FGF18 (Source: Uniprot.org, NCBI)
Gene Name
FGF18
Full Name
Fibroblast growth factor 18
Weight
23989 MW
Superfamily
heparin-binding growth factors family
Alternative Names
Fibroblast growth factor 18;FGF-18;zFGF5;FGF18;UNQ420/PRO856; FGF18 FGF-18, ZFGF5 fibroblast growth factor 18 fibroblast growth factor 18
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FGF18, check out the FGF18 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FGF18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FGF-18 Antibody (A04181-1)
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No publications found for A04181-1
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-FGF-18 Antibody
Question
Is this A04181-1 anti-FGF-18 antibody reactive to the isotypes of FGF18?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
The immunogen of A04181-1 anti-FGF-18 antibody is Synthesized peptide derived from the C-terminal region of human FGF-18. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-24
Question
I see that the anti-FGF-18 antibody A04181-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-19
Question
See below the WB image, lot number and protocol we used for ovary using anti-FGF-18 antibody A04181-1. Please let me know if you require anything else.
R. Zhao
Verified customer
Asked: 2020-03-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-18
Question
Our lab want to know about to test anti-FGF-18 antibody A04181-1 on rat ovary for research purposes, then I may be interested in using anti-FGF-18 antibody A04181-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
The products we sell, including anti-FGF-18 antibody A04181-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-12
Question
Would anti-FGF-18 antibody A04181-1 work for IHC with ovary?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
According to the expression profile of ovary, FGF18 is highly expressed in ovary. So, it is likely that anti-FGF-18 antibody A04181-1 will work for IHC with ovary.
Boster Scientific Support
Answered: 2020-01-08
Question
Is a blocking peptide available for product anti-FGF-18 antibody (A04181-1)?
P. Banerjee
Verified customer
Asked: 2019-11-12
Answer
We do provide the blocking peptide for product anti-FGF-18 antibody (A04181-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-12
Question
My question regarding product A04181-1, anti-FGF-18 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04181-1 anti-FGF-18 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-17
Question
Is there a BSA free version of anti-FGF-18 antibody A04181-1 available?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FGF-18 antibody A04181-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-11
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ovary using anti-FGF-18 antibody A04181-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-03
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-03
Question
We are currently using anti-FGF-18 antibody A04181-1 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-29
Answer
The anti-FGF-18 antibody (A04181-1) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-29
Question
Would A04181-1 anti-FGF-18 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-03-15
Answer
You can see on the product datasheet, A04181-1 anti-FGF-18 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-03-15
Question
I was wanting to use your anti-FGF-18 antibody for IHC for rat ovary on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat ovary identification?
E. Jackson
Verified customer
Asked: 2017-05-08
Answer
It shows on the product datasheet, A04181-1 anti-FGF-18 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-05-08
Question
Would anti-FGF-18 antibody A04181-1 work on pig IHC with lung?
K. Jha
Verified customer
Asked: 2015-06-08
Answer
Our lab technicians have not tested anti-FGF-18 antibody A04181-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-FGF-18 antibody A04181-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig lung in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-06-08
