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Product Info Summary
| SKU: | A02368-2 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-Ephrin-B2 (Ab-330) EFNB2 Antibody
SKU/Catalog Number
A02368-2
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Ephrin-B2 (Ab-330) EFNB2 Antibody (Catalog # A02368-2). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ephrin-B2 (Ab-330) EFNB2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02368-2)
Host
Rabbit
Contents
pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.328~332 (N-I-Y-Y-K) derived from Human Ephrin B (ephrin-B2).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02368-2 is reactive to EFNB2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
74270 MW
Background of Ephrin-B2
Ephrin-B2 encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a lycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors.
Chrencik JE,et al.( 2006) J Biol Chem;281(38):28185-28192.
Kertesz N, et al. (2006) Blood; 107(6):2330-2338.
Noren NK, et al. (2004) Proc Natl Acad Sci USA; 101(15):5583-5588.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02368-2 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 37kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Immunohistochemistry:1:100~1:200
Validation Images & Assay Conditions
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Immunofluorescent analysis of formalin-fixed, paraffin-embedded hela cells with A02368-2 at 1/75 dilution.A Goat Anti-Rabbit IgG H&L (CY3) at 1/50 was used as secondary.
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Immunofluorescent analysis of formalin-fixed, paraffin-embedded C6 cells with A02368-2 at 1/75 dilution.A Goat Anti-Rabbit IgG H&L (CY3) at 1/50 was used as secondary.
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All lanes : Ephrin-B2(Ab-330) Antibody(A02368-2) at 1/1000 dilutionLane 1 : Mouse spleen tissue
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Figure 2. IHC analysis of EFNB2 using anti-EFNB2 antibody (A02368-2).
EFNB2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-EFNB2 Antibody (A02368-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of EFNB2 using anti-EFNB2 antibody (A02368-2).
EFNB2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-EFNB2 Antibody (A02368-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of EFNB2 using anti-EFNB2 antibody (A02368-2).
EFNB2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-EFNB2 Antibody (A02368-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of EFNB2 using anti-EFNB2 antibody (A02368-2).
EFNB2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-EFNB2 Antibody (A02368-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For EFNB2 (Source: Uniprot.org, NCBI)
Gene Name
EFNB2
Full Name
Ephrin-B2
Weight
74270 MW
Superfamily
ephrin family
Alternative Names
Amiloride-sensitive sodium channel subunit gamma;Epithelial Na (+) channel subunit gamma;ENaCG;Gamma-ENaC;Gamma-NaCH;Nonvoltage-gated sodium channel 1 subunit gamma;SCNEG;SCNN1G; EFNB2 EPLG5, HTKL, Htk-L, LERK5 ephrin B2 ephrin-B2|HTK ligand|eph-related receptor tyrosine kinase ligand 5|ligand of eph-related kinase 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EFNB2, check out the EFNB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EFNB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ephrin-B2 (Ab-330) EFNB2 Antibody (A02368-2)
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Customer Q&As
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5 Customer Q&As for Anti-Ephrin-B2 (Ab-330) EFNB2 Antibody
Question
We are currently using anti-Ephrin-B2 (Ab-330) antibody A02368-2 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
The anti-Ephrin-B2 (Ab-330) antibody (A02368-2) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-30
Question
Would anti-Ephrin-B2 (Ab-330) antibody A02368-2 work for IF with brain?
Verified Customer
Verified customer
Asked: 2018-12-26
Answer
According to the expression profile of brain, EFNB2 is highly expressed in brain. So, it is likely that anti-Ephrin-B2 (Ab-330) antibody A02368-2 will work for IF with brain.
Boster Scientific Support
Answered: 2018-12-26
Question
Can you help my question with product A02368-2, anti-Ephrin-B2 (Ab-330) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-08-27
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02368-2 anti-Ephrin-B2 (Ab-330) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-27
Question
My lab would like to test anti-Ephrin-B2 (Ab-330) antibody A02368-2 on rat brain for research purposes, then I may be interested in using anti-Ephrin-B2 (Ab-330) antibody A02368-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Banerjee
Verified customer
Asked: 2018-04-05
Answer
The products we sell, including anti-Ephrin-B2 (Ab-330) antibody A02368-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-04-05
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Ephrin-B2 (Ab-330) antibody A02368-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-09-08
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-08
