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Product Info Summary
| SKU: | A01453 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-FES Antibody Picoband®
SKU/Catalog Number
A01453
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FES Antibody Picoband® catalog # A01453. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FES Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01453)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human FES, which shares 93.5% amino acid (aa) sequence identity with both mouse and rat FES.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01453 is reactive to FES in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
93 kDa
Calculated molecular weight
51561 MW
Background of FES
FES (feline sarcoma oncogene) is an enzyme that in humans is encoded by the FES gene. This gene encodes the human cellular counterpart of a feline sarcoma retrovirus protein with transforming capabilities. Non-onc intervening sequences were present in the human counterpart. The gene product has tyrosine-specific protein kinase activity and that activity is required for maintenance of cellular transformation. Its chromosomal location has linked it to a specific translocation event identified in patients with acute promyelocytic leukemia, but it is also involved in normal hematopoiesis. A truncated transcript has been identified that is generated utilizing a start site in one of the far downstream exons but a protein product associated with this transcript has not been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01453 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: human Hela cell, human 293T cell, human SK-OV-3 cell, human A549 cell, human MCF-7 cell, human placenta tissue, human PANC-1 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FES using anti-FES antibody (A01453).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela cell lysate,
Lane 2: human 293T cell lysate,
Lane 3: human SK-OV-3 cell lysate,
Lane 4: human A549 cell lysate,
Lane 5: human MCF-7 cell lysate,
Lane 6: human placenta tissue lysate,
Lane 7: human PANC-1 cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FES antigen affinity purified polyclonal antibody (Catalog # A01453) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FES at approximately 93KD. The expected band size for FES is at 93KD.
Protein Target Info & Infographic
Gene/Protein Information For FES (Source: Uniprot.org, NCBI)
Gene Name
FES
Full Name
Tyrosine-protein kinase Fes/Fps
Weight
51561 MW
Superfamily
protein kinase superfamily
Alternative Names
Tyrosine-protein kinase Fes/Fps; Feline sarcoma/Fujinami avian sarcoma oncogene homolog; Proto-oncogene c-Fes; Proto-oncogene c-Fps; p93c-fes; FES; FPS FES FPS FES proto-oncogene, tyrosine kinase tyrosine-protein kinase Fes/Fps|Oncogene FES, feline sarcoma virus|feline sarcoma (Snyder-Theilen) viral (v-fes)/Fujinami avian sarcoma (PRCII) viral (v-fps) oncogene homolog|feline sarcoma oncogene|feline sarcoma/Fujinami avian sarcoma oncogene homolog|p93c-fes|proto-oncogene c-Fes|proto-oncogene c-Fps|proto-oncogene tyrosine-protein kinase Fes/Fps
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FES, check out the FES Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FES: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FES Antibody Picoband® (A01453)
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