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Product Info Summary
| SKU: | A04086 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-FBXO7 Antibody
SKU/Catalog Number
A04086
Size
100μl
Form
Liquid
Description
Boster Bio Anti-FBXO7 Antibody catalog # A04086. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FBXO7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04086)
Host
Rabbit
Contents
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide from human protein at AA range: 371-420
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04086 is reactive to FBXO7 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
58503 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04086 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:5000-10000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of Mouse Kidney cells using antibody diluted at 1:1000.
Protein Target Info & Infographic
Gene/Protein Information For FBXO7 (Source: Uniprot.org, NCBI)
Gene Name
FBXO7
Full Name
F-box only protein 7
Weight
58503 MW
Alternative Names
F-box only protein 7;FBXO7;FBX7; FBXO7 FBX, FBX07, FBX7, PARK15, PKPS F-box protein 7 F-box only protein 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FBXO7, check out the FBXO7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FBXO7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FBXO7 Antibody (A04086)
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No publications found for A04086
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-FBXO7 Antibody
Question
Can you help my question with product A04086, anti-FBXO7 antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04086 anti-FBXO7 antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-08
Question
Does A04086 anti-FBXO7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
As indicated on the product datasheet, A04086 anti-FBXO7 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-06
Question
Is a blocking peptide available for product anti-FBXO7 antibody (A04086)?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
We do provide the blocking peptide for product anti-FBXO7 antibody (A04086). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-10
Question
We need to test anti-FBXO7 antibody A04086 on human bone marrow for research purposes, then I may be interested in using anti-FBXO7 antibody A04086 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
The products we sell, including anti-FBXO7 antibody A04086, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-10
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow using anti-FBXO7 antibody A04086. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-30
Question
I was wanting to use your anti-FBXO7 antibody for WB for human bone marrow on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human bone marrow identification?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
As indicated on the product datasheet, A04086 anti-FBXO7 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human bone marrow in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-17
Question
Please see the WB image, lot number and protocol we used for bone marrow using anti-FBXO7 antibody A04086. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-12-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-04
Question
I see that the anti-FBXO7 antibody A04086 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-05-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-05-11
Question
Will anti-FBXO7 antibody A04086 work on feline WB with heart testis?
Verified Customer
Verified customer
Asked: 2018-01-01
Answer
Our lab technicians have not validated anti-FBXO7 antibody A04086 on feline. You can run a BLAST between feline and the immunogen sequence of anti-FBXO7 antibody A04086 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline heart testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-01
Question
Will anti-FBXO7 antibody A04086 work for WB with bone marrow?
Verified Customer
Verified customer
Asked: 2017-12-07
Answer
According to the expression profile of bone marrow, FBXO7 is highly expressed in bone marrow. So, it is likely that anti-FBXO7 antibody A04086 will work for WB with bone marrow.
Boster Scientific Support
Answered: 2017-12-07
Question
We are currently using anti-FBXO7 antibody A04086 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-06
Answer
The anti-FBXO7 antibody (A04086) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-06
Question
Is this A04086 anti-FBXO7 antibody reactive to the isotypes of FBXO7?
Verified Customer
Verified customer
Asked: 2017-05-26
Answer
The immunogen of A04086 anti-FBXO7 antibody is Synthetic peptide from AA range: 371-420. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-05-26
Question
Is there a BSA free version of anti-FBXO7 antibody A04086 available?
G. Taylor
Verified customer
Asked: 2013-11-14
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-FBXO7 antibody A04086 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-11-14
