Product Info Summary
SKU: | A09728-1 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Cysteine protease ATG4C ATG4C Antibody
SKU/Catalog Number
A09728-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Cysteine protease ATG4C ATG4C Antibody (Catalog # A09728-1). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cysteine protease ATG4C ATG4C Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09728-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ATG4C.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09728-1 is reactive to ATG4C in Human, Mouse
Reconstitution
Calculated molecular weight
52497 MW
Background of ATG4C
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation By similarity. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Marino G., J. Biol. Chem. 278:3671-3678(2003).
Chen J.M., Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
Ota T., Nat. Genet. 36:40-45(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09728-1 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using ATG4C antibody A09728-1.
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Western blot analysis of extracts from K562 cells and COS7 cells, using ATG4C antibody A09728-1.
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Immunofluorescence analysis of HuvEc cells, using ATG4C antibody A09728-1.
Protein Target Info & Infographic
Gene/Protein Information For ATG4C (Source: Uniprot.org, NCBI)
Gene Name
ATG4C
Full Name
Cysteine protease ATG4C
Weight
52497 MW
Superfamily
peptidase C54 family
Alternative Names
Cysteine protease ATG4C;3.4.22.-;AUT-like 3 cysteine endopeptidase;Autophagin-3;Autophagy-related cysteine endopeptidase 3;Autophagy-related protein 4 homolog C;ATG4C;APG4C, AUTL1, AUTL3; ATG4C APG4-C, APG4C, AUTL1, AUTL3 autophagy related 4C cysteine peptidase cysteine protease ATG4C|APG4 autophagy 4 homolog C|ATG4 autophagy related 4 homolog C|AUT-like 1, cysteine endopeptidase|AUT-like 3 cysteine endopeptidase|autophagin-3|autophagy-related cysteine endopeptidase 3|autophagy-related protein 4 homolog C|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATG4C, check out the ATG4C Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATG4C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cysteine protease ATG4C ATG4C Antibody (A09728-1)
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