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Product Info Summary
| SKU: | A01377-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-FBP1 Antibody Picoband®
SKU/Catalog Number
A01377-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FBP1 Antibody Picoband® catalog # A01377-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-FBP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01377-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human FBP1 recombinant protein (Position: M1-A44).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01377-1 is reactive to FBP1 in Human, Mouse
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
37 kDa
Calculated molecular weight
36.842kDa
Background of FBPase 1
Fructose-1,6-bisphosphatase 1 is a protein that in humans is encoded by the FBP1 gene. Fructose-1,6-bisphosphatase 1, a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1,6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01377-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HCCT tissue, mouse liver tissue, mouse kidney tissue
ICC/IF: MCF-7 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FBP1 using anti-FBP1 antibody (A01377-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HCCT tissue lysates,
Lane 2: mouse liver tissue lysates,
Lane 3: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBP1 antigen affinity purified polyclonal antibody (Catalog # A01377-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FBP1 at approximately 37 kDa. The expected band size for FBP1 is at 37 kDa.
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Figure 2. IF analysis of FBP1 using anti-FBP1 antibody (A01377-1).
FBP1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FBP1 Antibody (A01377-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For FBP1 (Source: Uniprot.org, NCBI)
Gene Name
FBP1
Full Name
Fructose-1,6-bisphosphatase 1
Weight
36.842kDa
Superfamily
FBPase class 1 family
Alternative Names
DNA polymerase iota; Eta2; RAD30 homolog B; POLI; RAD30B FBP1 FBP fructose-bisphosphatase 1 fructose-1,6-bisphosphatase 1|D-fructose-1,6-bisphosphate 1-phosphohydrolase 1|FBPase 1|growth-inhibiting protein 17|liver FBPase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FBP1, check out the FBP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FBP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FBP1 Antibody Picoband® (A01377-1)
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