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Product Info Summary
| SKU: | A00941 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Fatty Acid Synthase(FASN) Antibody
View all Fatty Acid Synthase/FASN Antibodies
SKU/Catalog Number
A00941
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Fatty Acid Synthase(FASN) Antibody (Catalog # A00941). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fatty Acid Synthase(FASN) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00941)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.2503~2507 (E-P-R-V-S) derived from Human Fatty Acid Synthase (FASN).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00941 is reactive to FASN in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
273427 MW
Background of Fatty Acid Synthase/FASN
Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.
Jayakumar A., Tai M.-H., Huang W.-Y. Natl. Acad. Sci. U.S.A. 92:8695-8699(1995)
Chi A., Valencia J.C., Hu Z.-Z., Watabe H. Proteome Res. 5:3135-3144(2006)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00941 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 273kd
Western blotting: 1:500~1:1000
Validation Images & Assay Conditions
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Western blot analysis of extract from Rat brain tissue, Hela, Raji, P19 cells using Fatty Acid Synthase(FASN) antibody A00941
Protein Target Info & Infographic
Gene/Protein Information For FASN (Source: Uniprot.org, NCBI)
Gene Name
FASN
Full Name
Fatty acid synthase
Weight
273427 MW
Alternative Names
Fatty acid synthase;2.3.1.85;[Acyl-carrier-protein] S-acetyltransferase;2.3.1.38;[Acyl-carrier-protein] S-malonyltransferase;2.3.1.39;3-oxoacyl-[acyl-carrier-protein] synthase;2.3.1.41;3-oxoacyl-[acyl-carrier-protein] reductase;1.1.1.100;3-hydroxyacyl-[acyl-carrier-protein] dehydratase;4.2.1.59;Enoyl-[acyl-carrier-protein] reductase;1.3.1.39;Oleoyl-[acyl-carrier-protein] hydrolase;3.1.2.14;FASN;FAS; FASN FAS, OA-519, SDR27X1 fatty acid synthase fatty acid synthase|short chain dehydrogenase/reductase family 27X, member 1|type I fatty acid synthase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FASN, check out the FASN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FASN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fatty Acid Synthase(FASN) Antibody (A00941)
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No publications found for A00941
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Fatty Acid Synthase(FASN) Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Fatty Acid Synthase(FASN) antibody A00941. Let me know if you need anything else.
K. Mangal
Verified customer
Asked: 2020-01-24
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-24
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma using anti-Fatty Acid Synthase(FASN) antibody A00941. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-19
Question
I was wanting to use your anti-Fatty Acid Synthase(FASN) antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix carcinoma identification?
K. Williams
Verified customer
Asked: 2019-11-19
Answer
It shows on the product datasheet, A00941 anti-Fatty Acid Synthase(FASN) antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-19
Question
Our team were happy with the WB result of your anti-Fatty Acid Synthase(FASN) antibody. However we have been able to see positive staining in brain cytoplasm. using this antibody. Is that expected? Could you tell me where is FASN supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
From what I have seen in literature, brain does express FASN. Generally FASN expresses in cytoplasm. Regarding which tissues have FASN expression, here are a few articles citing expression in various tissues:
B-cell lymphoma, Pubmed ID: 8022791
Brain, Pubmed ID: 7567999, 16625196
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Eye, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Boster Scientific Support
Answered: 2019-11-11
Question
I have a question about product A00941, anti-Fatty Acid Synthase(FASN) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00941 anti-Fatty Acid Synthase(FASN) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-05
Question
Will A00941 anti-Fatty Acid Synthase(FASN) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
You can see on the product datasheet, A00941 anti-Fatty Acid Synthase(FASN) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-10
Question
I see that the anti-Fatty Acid Synthase(FASN) antibody A00941 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-02
Question
My lab would like using your anti-Fatty Acid Synthase(FASN) antibody for neutrophil differentiation studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
We appreciate your inquiry. This A00941 anti-Fatty Acid Synthase(FASN) antibody is validated on rat brain tissue, raji p19 cells. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-07-09
Question
Would anti-Fatty Acid Synthase(FASN) antibody A00941 work for WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-01-23
Answer
According to the expression profile of cervix carcinoma, FASN is highly expressed in cervix carcinoma. So, it is likely that anti-Fatty Acid Synthase(FASN) antibody A00941 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2019-01-23
Question
Is a blocking peptide available for product anti-Fatty Acid Synthase(FASN) antibody (A00941)?
Verified Customer
Verified customer
Asked: 2018-03-29
Answer
We do provide the blocking peptide for product anti-Fatty Acid Synthase(FASN) antibody (A00941). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-03-29
Question
We are currently using anti-Fatty Acid Synthase(FASN) antibody A00941 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
M. Krishna
Verified customer
Asked: 2017-10-23
Answer
The anti-Fatty Acid Synthase(FASN) antibody (A00941) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-23
Question
We are interested in to test anti-Fatty Acid Synthase(FASN) antibody A00941 on mouse cervix carcinoma for research purposes, then I may be interested in using anti-Fatty Acid Synthase(FASN) antibody A00941 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-25
Answer
The products we sell, including anti-Fatty Acid Synthase(FASN) antibody A00941, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-25
Question
We have been able to see staining in mouse cervix carcinoma erythroleukemia. Are there any suggestions? Is anti-Fatty Acid Synthase(FASN) antibody supposed to stain cervix carcinoma erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2017-06-05
Answer
From what I have seen in literature cervix carcinoma erythroleukemia does express FASN. From what I have seen in Uniprot.org, FASN is expressed in liver, brain, eye, b-cell lymphoma, lymphoblast, melanoma, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have FASN expression, here are a few articles citing expression in various tissues:
B-cell lymphoma, Pubmed ID: 8022791
Brain, Pubmed ID: 7567999, 16625196
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Eye, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Boster Scientific Support
Answered: 2017-06-05
Question
Is this A00941 anti-Fatty Acid Synthase(FASN) antibody reactive to the isotypes of FASN?
K. Parker
Verified customer
Asked: 2016-04-29
Answer
The immunogen of A00941 anti-Fatty Acid Synthase(FASN) antibody is Peptide sequence around aa.2503~2507 (E-P-R-V-S) derived from Human Fatty Acid Synthase (FASN). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-04-29
Question
Do you have a BSA free version of anti-Fatty Acid Synthase(FASN) antibody A00941 available?
G. Miller
Verified customer
Asked: 2013-12-23
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Fatty Acid Synthase(FASN) antibody A00941 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-12-23
